PMID- 18450956
OWN - NLM
STAT- MEDLINE
DCOM- 20080826
LR  - 20211020
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 149
IP  - 8
DP  - 2008 Aug
TI  - Pituitary adenylate cyclase-activating polypeptide regulates brain-derived 
      neurotrophic factor exon IV expression through the VPAC1 receptor in the 
      amphibian melanotrope cell.
PG  - 4177-82
LID - 10.1210/en.2008-0131 [doi]
AB  - In mammals, pituitary adenylate cyclase-activating polypeptide (PACAP) and its 
      receptors PAC1-R, VPAC1-R, and VPAC2-R play a role in various physiological 
      processes, including proopiomelanocortin (POMC) and brain-derived neurotrophic 
      factor (BDNF) gene expression. We have previously found that PACAP stimulates 
      POMC gene expression, POMC biosynthesis, and alpha-MSH secretion in the 
      melanotrope cell of the amphibian Xenopus laevis. This cell hormonally controls 
      the process of skin color adaptation to background illumination. Here, we have 
      tested the hypothesis that PACAP is involved in the regulation of Xenopus 
      melanotrope cell activity during background adaptation and that part of this 
      regulation is through the control of the expression of autocrine acting BDNF. 
      Using quantitative RT-PCR, we have identified the Xenopus PACAP receptor, 
      VPAC1-R, and show that this receptor in the melanotrope cell is under strong 
      control of the background light condition, whereas expression of PAC1-R was 
      absent from these cells. Moreover, we reveal by quantitative immunocytochemistry 
      that the neural pituitary lobe of white-background adapted frogs possesses a much 
      higher PACAP content than the neural lobe of black-background adapted frogs, 
      providing evidence that PACAP produced in the hypothalamic magnocellular nucleus 
      plays an important role in regulating the activity of Xenopus melanotrope cells 
      during background adaptation. Finally, an in vitro study demonstrates that PACAP 
      stimulates the expression of BDNF transcript IV.
FAU - Kidane, Adhanet H
AU  - Kidane AH
AD  - Department of Cellular Animal Physiology, Donders Centre for Neuroscience, 
      European Graduate School of Neuroscience, Faculty of Science, Radboud University 
      Nijmegen, 6500 GL Nijmegen, The Netherlands.
FAU - Roubos, Eric W
AU  - Roubos EW
FAU - Jenks, Bruce G
AU  - Jenks BG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080501
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Vasoactive Intestinal Polypeptide, Type I)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cloning, Molecular
MH  - *Exons
MH  - Gene Expression Regulation/drug effects
MH  - Melanotrophs/*metabolism
MH  - Pituitary Adenylate Cyclase-Activating 
      Polypeptide/metabolism/pharmacology/*physiology
MH  - RNA, Messenger/metabolism
MH  - Receptors, Vasoactive Intestinal Polypeptide, Type 
      I/genetics/metabolism/*physiology
MH  - Tissue Distribution
MH  - Xenopus laevis/*genetics
PMC - PMC2488213
EDAT- 2008/05/03 09:00
MHDA- 2008/08/30 09:00
PMCR- 2009/08/01
CRDT- 2008/05/03 09:00
PHST- 2008/05/03 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/05/03 09:00 [entrez]
PHST- 2009/08/01 00:00 [pmc-release]
AID - en.2008-0131 [pii]
AID - 4192 [pii]
AID - 10.1210/en.2008-0131 [doi]
PST - ppublish
SO  - Endocrinology. 2008 Aug;149(8):4177-82. doi: 10.1210/en.2008-0131. Epub 2008 May 
      1.