PMID- 18451507
OWN - NLM
STAT- MEDLINE
DCOM- 20080619
LR  - 20190720
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 31
IP  - 5
DP  - 2008 May
TI  - Altered levels of oxidation and phospholipase C isozyme expression in the brains 
      of theanine-administered rats.
PG  - 857-60
AB  - Previously, we revealed that theanine, a green tea component, induced 
      phospholipase C (PLC)-beta1 and -gamma1, stress-responsible molecules, in primary 
      cultured rat cerebral cortical neurons, suggesting its protective effect on 
      oxidative stress in neurons. In this study, we investigated whether the same 
      favorable effect occurs in vivo. On the oral administration of theanine (10 mmol 
      (1.74 g)/kg, once a day) to rats via gastric intubation for 2 weeks, there was no 
      change in the weight of the body or the cerebral cortex (Cx), cerebellum (Cb), or 
      hippocampus (Hip) in the brain. On assessment of oxidation levels in the brain 
      with thiobarbiturate reactive substances as a marker, the levels were found to be 
      20% lower in the Cx of theanine-treated rats than in that of control ones. The 
      protein expression levels of PLC-beta1 and -gamma1 were significantly increased 
      in the Cx on theanine administration and the same tendency was observed in the 
      Cb, but not the Hip. In addition, the protein expression level of PLC-delta1, 
      which plays an opposite role to the other two isozymes, was not affected in any 
      brain regions on theanine administration. Overall, it was demonstrated that 
      theanine is a safe compound and its repeated oral administration reduces 
      oxidation levels in the brain, especially the Cx, by increasing PLC-beta1 and 
      -gamma1 protein expression, suggesting its favorable effect on the brain in vivo.
FAU - Nishida, Kentaro
AU  - Nishida K
AD  - Department of Environmental Biochemistry, Kyoto Pharmaceutical University, 5 
      Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
FAU - Yasuda, Eri
AU  - Yasuda E
FAU - Nagasawa, Kazuki
AU  - Nagasawa K
FAU - Fujimoto, Sadaki
AU  - Fujimoto S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Glutamates)
RN  - 0 (Isoenzymes)
RN  - 8021PR16QO (theanine)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Body Weight/drug effects
MH  - Brain/drug effects/*enzymology
MH  - Brain Chemistry/*drug effects
MH  - Catalase/metabolism
MH  - Glutamates/*pharmacology/toxicity
MH  - Isoenzymes/biosynthesis
MH  - Male
MH  - Organ Size/drug effects
MH  - Oxidation-Reduction
MH  - Rats
MH  - Rats, Wistar
MH  - Type C Phospholipases/*biosynthesis
EDAT- 2008/05/03 09:00
MHDA- 2008/06/20 09:00
CRDT- 2008/05/03 09:00
PHST- 2008/05/03 09:00 [pubmed]
PHST- 2008/06/20 09:00 [medline]
PHST- 2008/05/03 09:00 [entrez]
AID - JST.JSTAGE/bpb/31.857 [pii]
AID - 10.1248/bpb.31.857 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2008 May;31(5):857-60. doi: 10.1248/bpb.31.857.