PMID- 18452135
OWN - NLM
STAT- MEDLINE
DCOM- 20081104
LR  - 20131121
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 23
IP  - 5
DP  - 2008 Sep-Oct
TI  - Photo-induced chemiluminescent method for determination of reducing sugars.
PG  - 287-91
LID - 10.1002/bio.1030 [doi]
AB  - In the present study, a simple and sensitive photo-induced chemiluminescence (CL) 
      method for the quantitation of reducing sugars, including fructose, glucose, 
      sucrose and lactose, was developed. This method was based on the on-line 
      photocatalytic reaction of the reducing sugars, using a home-made photoreactor 
      consisting of PTEF tube helically coiling around a high-pressure mercury UV lamp. 
      Reducing sugars were detected by direct CL emission resulting from the reaction 
      between the photoproducts and luminol. To maximize the signal intensity, the 
      effects of irradiation time, flow rate, luminol concentration, buffer pH and 
      concentration were tested. Under optimum conditions, the linear dynamic ranges 
      were all 0.36-18 mg/L and the relative standard deviations (RSDs) were 1.8-2.3%, 
      with limits of detection (3sigma) of 0.06 mg/L for fructose, glucose, sucrose and 
      lactose. Finally, interference effects from ascorbic acid, amino acids (alanine, 
      glycine, serine, leucine and methionine) and some metal ions and anions were also 
      investigated.
FAU - Chen, He
AU  - Chen H
AD  - Key Laboratory of Green Chemistry and Technology, College of Chemistry, Sichuan 
      University, Chengdu, Sichuan 610064, People's Republic of China.
FAU - Gao, Ying
AU  - Gao Y
FAU - Su, Yingying
AU  - Su Y
FAU - Lv, Yi
AU  - Lv Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Carbohydrates)
RN  - 30237-26-4 (Fructose)
RN  - 57-50-1 (Sucrose)
RN  - 5EXP385Q4F (Luminol)
RN  - IY9XDZ35W2 (Glucose)
RN  - J2B2A4N98G (Lactose)
SB  - IM
MH  - Carbohydrates/*analysis
MH  - Equipment Design
MH  - Fructose/analysis
MH  - Glucose/analysis
MH  - Lactose/analysis
MH  - Luminescent Measurements/*methods/standards
MH  - Luminol
MH  - Photochemistry
MH  - Sucrose/analysis
EDAT- 2008/05/03 09:00
MHDA- 2008/11/05 09:00
CRDT- 2008/05/03 09:00
PHST- 2008/05/03 09:00 [pubmed]
PHST- 2008/11/05 09:00 [medline]
PHST- 2008/05/03 09:00 [entrez]
AID - 10.1002/bio.1030 [doi]
PST - ppublish
SO  - Luminescence. 2008 Sep-Oct;23(5):287-91. doi: 10.1002/bio.1030.