PMID- 18452227
OWN - NLM
STAT- MEDLINE
DCOM- 20080909
LR  - 20131121
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 8
IP  - 11
DP  - 2008 Jun
TI  - Proteomic and transcriptomic analysis for streptozotocin-induced diabetic rat 
      pancreas in response to fungal polysaccharide treatments.
PG  - 2344-61
LID - 10.1002/pmic.200700779 [doi]
AB  - In an attempt to search for novel biomarkers for monitoring diabetes prognosis, 
      we examined the influence of the hypoglycemic fungal extracellular 
      polysaccharides (EPS) on the differential change in pancreatic proteome and 
      transcriptome in streptozotocin (STZ)-induced diabetic rats using 2-DE-based 
      protein mapping and oligonucleotide microarray analysis. The 2-DE system 
      separated more than 2000 individual spots, demonstrating that 34 proteins out of 
      about 500 matched spots were differentially expressed. A total of 22 
      overexpressed and 12 underexpressed proteins in 2-DE map were observed (p<0.05) 
      between the healthy and diabetic rats, of which 26 spots were identified by PMF 
      analysis. Of these, significant down regulation of carbonyl reductase (Cbr), 
      hydroxymethylglutaryl-CoA synthase (HMGCS), and putative human mitogen-activated 
      protein kinase activator with WD repeats-binding protein (MAWDBP) in diabetic 
      pancreas were reported for the first time in this study. When treated with EPS, 
      all these four proteins were reverted to normal levels. The microarray analysis 
      revealed that 96 out of 1272 genes were down- or up-regulated in the diabetic 
      rats and the altered transcript levels of many of these genes were reversed after 
      EPS treatment. In particular, ROS generation in rat islets was significantly 
      increased after STZ treatment, thereafter EPS treatment was likely to play a 
      preventive role in beta-cell destruction mediated by STZ. Taken together, EPS may 
      act as a potent regulator of gene expression for a wide variety of genes in 
      diabetic rats, particularly in antioxidative stress, insulin biosynthesis, and 
      cell proliferation.
FAU - Kim, Sang Woo
AU  - Kim SW
AD  - Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Korea.
FAU - Hwang, Hye Jin
AU  - Hwang HJ
FAU - Baek, Yu Mi
AU  - Baek YM
FAU - Lee, Sung Hak
AU  - Lee SH
FAU - Hwang, Hee Sun
AU  - Hwang HS
FAU - Yun, Jong Won
AU  - Yun JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Antioxidants)
RN  - 0 (Polysaccharides)
RN  - 0 (Reactive Oxygen Species)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - *Gene Expression Regulation
MH  - Islets of Langerhans/cytology
MH  - MAP Kinase Signaling System
MH  - Male
MH  - Pancreas/*metabolism
MH  - Polysaccharides/*chemistry
MH  - Proteomics/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species
MH  - Streptozocin/pharmacology
MH  - *Transcription, Genetic
EDAT- 2008/05/03 09:00
MHDA- 2008/09/10 09:00
CRDT- 2008/05/03 09:00
PHST- 2008/05/03 09:00 [pubmed]
PHST- 2008/09/10 09:00 [medline]
PHST- 2008/05/03 09:00 [entrez]
AID - 10.1002/pmic.200700779 [doi]
PST - ppublish
SO  - Proteomics. 2008 Jun;8(11):2344-61. doi: 10.1002/pmic.200700779.