PMID- 18452993
OWN - NLM
STAT- MEDLINE
DCOM- 20081105
LR  - 20161124
IS  - 0161-813X (Print)
IS  - 0161-813X (Linking)
VI  - 29
IP  - 4
DP  - 2008 Jul
TI  - Protective effect of the green tea component, L-theanine on environmental 
      toxins-induced neuronal cell death.
PG  - 656-62
LID - 10.1016/j.neuro.2008.03.004 [doi]
AB  - Several environmental neurotoxins and oxidative stress inducers are known to 
      damage the nervous system and are considered major factors associated with the 
      selective vulnerability of nigral dopaminergic neurons in Parkinson's disease 
      (PD). Gamma-glutamylethylamide (L-theanine), a natural glutamate analog in green 
      tea, has been shown to exert strong anti-ischemic effect. In this study, we 
      investigated the protective effects of L-theanine on neurotoxicity induced by 
      PD-related neurotoxicants, rotenone and dieldrin in cultured human dopaminergic 
      cell line, SH-SY5Y. Our initial experiments revealed that L-theanine (500 microM) 
      attenuated both rotenone- and dieldrin-induced DNA fragmentation and apoptotic 
      death in SH-SY5Y cells. In addition, L-theanine partially prevented both 
      rotenone- and dieldrin-induced heme oxygenase-1 (HO-1) up-regulation. Both 
      rotenone- and dieldrin-induced down-regulation of extracellular signal-regulated 
      kinase1/2 (ERK1/2) phosphorylation was significantly blocked by pretreatment with 
      L-theanine. Furthermore, pretreatment with L-theanine significantly attenuated 
      the down-regulation of brain-derived neurotrophic factor (BDNF) and glial cell 
      line-derived neurotrophic factor (GDNF) production in SH-SY5Y cells. These 
      results suggest that L-theanine directly provide neuroprotection against 
      PD-related neurotoxicants and may be clinically useful for preventing PD 
      symptoms.
FAU - Cho, Hong-Suk
AU  - Cho HS
AD  - Department of Biotechnology BK21 Program, Chosun University, Gwangju, Republic of 
      Korea.
FAU - Kim, Seung
AU  - Kim S
FAU - Lee, Sook-Young
AU  - Lee SY
FAU - Park, Jeong Ae
AU  - Park JA
FAU - Kim, Sung-Jun
AU  - Kim SJ
FAU - Chun, Hong Sung
AU  - Chun HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080320
PL  - Netherlands
TA  - Neurotoxicology
JT  - Neurotoxicology
JID - 7905589
RN  - 0 (Glutamates)
RN  - 0 (Indoles)
RN  - 0 (Insecticides)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 03L9OT429T (Rotenone)
RN  - 47165-04-8 (DAPI)
RN  - 8021PR16QO (theanine)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - I0246D2ZS0 (Dieldrin)
SB  - IM
MH  - Analysis of Variance
MH  - Apoptosis/drug effects
MH  - Cell Line, Transformed
MH  - Dieldrin/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Glutamates/*pharmacology
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Indoles
MH  - Insecticides/*toxicity
MH  - Nerve Growth Factors/metabolism
MH  - Neurons/*drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Rotenone/*toxicity
EDAT- 2008/05/03 09:00
MHDA- 2008/11/06 09:00
CRDT- 2008/05/03 09:00
PHST- 2008/01/29 00:00 [received]
PHST- 2008/03/11 00:00 [revised]
PHST- 2008/03/11 00:00 [accepted]
PHST- 2008/05/03 09:00 [pubmed]
PHST- 2008/11/06 09:00 [medline]
PHST- 2008/05/03 09:00 [entrez]
AID - S0161-813X(08)00043-0 [pii]
AID - 10.1016/j.neuro.2008.03.004 [doi]
PST - ppublish
SO  - Neurotoxicology. 2008 Jul;29(4):656-62. doi: 10.1016/j.neuro.2008.03.004. Epub 
      2008 Mar 20.