PMID- 18456222
OWN - NLM
STAT- MEDLINE
DCOM- 20080812
LR  - 20080526
IS  - 0001-706X (Print)
IS  - 0001-706X (Linking)
VI  - 106
IP  - 3
DP  - 2008 Jun
TI  - Morphological and isoenzymatic differentiation of Phlebotomus perniciosus and 
      Phlebotomus longicuspis (Diptera: Psychodidae) in Southern Morocco.
PG  - 184-9
LID - 10.1016/j.actatropica.2008.03.011 [doi]
AB  - Morphological and enzymatic characterization of Phlebotomus perniciosus and 
      Phlebotomus longicuspis in Morocco is reported. Twenty-nine localities in central 
      and southern of Morocco were sampled and compared with three localities from the 
      Rif (northern Morocco). For morphological study, sand flies were collected by 
      sticky-paper traps. For males, the morphology of the copulatory valves (aedeagi) 
      was examined and the number of coxite hairs was recorded. For isoenzyme analyses, 
      specimens were collected in CDC light traps and immediately conserved at -80 
      degrees C. P. perniciosus samples from the south of Morocco, up to 150 km from 
      Marrakech, showed single-pointed aedeagi curved at their apices, 
      indistinguishable from the atypical morph of P. perniciosus, previously described 
      in northern Morocco. Twelve enzyme systems were tested and the qualitative 
      analysis of zymogram profiles revealed eight polymorphic loci (glucosephosphate 
      isomerase (GPI), phosphoglucomutase (PGM), hexokinase (HK), fumarate hydratase 
      (FUM), malate dehydrogenase 1 (MDH1), malate dehydrogenase 2 (MDH2), 6 
      phosphogluconate dehydrogenase (6PGD) and aconitase (ACO)). Enzyme loci showed 
      fixed alleles diagnostic for P. perniciosus (aconitase) and P. longicuspis 
      (aconitase and hexokinase).
FAU - Boussaa, S
AU  - Boussaa S
AD  - Equipe d'Ecologie Animale-Environnement, Faculte des Sciences Semlalia, 
      Universite Cadi Ayyad, Marrakech, Morocco. samia.boussaa@pharma.u-strasbg.fr
FAU - Boumezzough, A
AU  - Boumezzough A
FAU - Remy, P E
AU  - Remy PE
FAU - Glasser, N
AU  - Glasser N
FAU - Pesson, B
AU  - Pesson B
LA  - eng
PT  - Journal Article
DEP - 20080401
PL  - Netherlands
TA  - Acta Trop
JT  - Acta tropica
JID - 0370374
RN  - 0 (Isoenzymes)
SB  - IM
MH  - Animal Structures/*anatomy & histology
MH  - Animals
MH  - Cluster Analysis
MH  - Isoenzymes/*analysis/genetics
MH  - Male
MH  - Morocco
MH  - Phlebotomus/anatomy & histology/*classification/enzymology
EDAT- 2008/05/06 09:00
MHDA- 2008/08/13 09:00
CRDT- 2008/05/06 09:00
PHST- 2007/10/21 00:00 [received]
PHST- 2008/01/03 00:00 [revised]
PHST- 2008/03/19 00:00 [accepted]
PHST- 2008/05/06 09:00 [pubmed]
PHST- 2008/08/13 09:00 [medline]
PHST- 2008/05/06 09:00 [entrez]
AID - S0001-706X(08)00075-2 [pii]
AID - 10.1016/j.actatropica.2008.03.011 [doi]
PST - ppublish
SO  - Acta Trop. 2008 Jun;106(3):184-9. doi: 10.1016/j.actatropica.2008.03.011. Epub 
      2008 Apr 1.