PMID- 18457008
OWN - NLM
STAT- MEDLINE
DCOM- 20080910
LR  - 20211020
IS  - 1138-7548 (Print)
IS  - 1138-7548 (Linking)
VI  - 63
IP  - 4
DP  - 2007 Dec
TI  - Caffeine reduces TNFalpha up-regulation in human adipose tissue primary culture.
PG  - 329-36
AB  - Adipose tissue secretions play an important role in the development of 
      obesity-related pathologies such as diabetes. Through inflammatory cytokines 
      production, adipose tissue stromavascular fraction cells (SVF), and essentially 
      macrophages, promote adipocyte insulin resistance by a paracrine way. Since 
      xanthine family compounds such as caffeine were shown to decrease inflammatory 
      production by human blood cells, we investigated the possible effect of caffeine 
      on Tumor Necrosis Factor alpha (TNFalpha) and Interleukin-6 (IL-6) expression by 
      human adipose tissue primary culture. For that purpose, human subcutaneous 
      adipose tissue obtained from healthy non-obese women (BMI: 26.7 +/- 2.2 kg/m2) 
      after abdominal dermolipectomy, was split into explants and cultured for 6 hours 
      with or without caffeine. Three different concentrations of caffeine were tested 
      (0.5 microg/mL, 5 microg/mL and 50 microg/mL). After 6 hours of treatment, 
      explants were subjected to collagenase digestion in order to isolate adipocytes 
      and SVF cells. Then, TNFalpha and IL-6 mRNA were analysed by real-time PCR 
      alternatively in adipocytes and SVF cells. In parallel, we checked gene 
      expression of markers involved in adipocyte differenciation and in SVF cells 
      inflammation and proliferation. Our findings show a strong and dose dependent 
      down-regulation of TNF-alpha gene expression in both adipocyte and SVF cells 
      whereas IL-6 was only down regulated in SVF cells. No effect of caffeine was 
      noticed on the other genes studied. Thus, caffeine, by decreasing TNFalpha 
      expression, could improve adipose tissue inflammation during obesity.
FAU - Dray, C
AU  - Dray C
AD  - Institut National de la Sante et de la Recherche Medicale (INSERM), U858, 
      Toulouse, France. draycedr@toulouse.inserm.fr
FAU - Daviaud, D
AU  - Daviaud D
FAU - Guigne, C
AU  - Guigne C
FAU - Valet, P
AU  - Valet P
FAU - Castan-Laurell, I
AU  - Castan-Laurell I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - J Physiol Biochem
JT  - Journal of physiology and biochemistry
JID - 9812509
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Adipocytes/metabolism
MH  - Body Mass Index
MH  - Caffeine/*pharmacology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - RNA, Messenger/metabolism
MH  - Subcutaneous Fat/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/*genetics/metabolism
MH  - *Up-Regulation/drug effects
EDAT- 2008/05/07 09:00
MHDA- 2008/09/11 09:00
CRDT- 2008/05/07 09:00
PHST- 2008/05/07 09:00 [pubmed]
PHST- 2008/09/11 09:00 [medline]
PHST- 2008/05/07 09:00 [entrez]
AID - 10.1007/BF03165764 [doi]
PST - ppublish
SO  - J Physiol Biochem. 2007 Dec;63(4):329-36. doi: 10.1007/BF03165764.