PMID- 18458058
OWN - NLM
STAT- MEDLINE
DCOM- 20080715
LR  - 20211020
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 28
IP  - 14
DP  - 2008 Jul
TI  - Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 
      involved in mitotic control.
PG  - 4629-41
LID - 10.1128/MCB.00120-08 [doi]
AB  - Nuclear factor 90 (NF90) and its C-terminally extended isoform, NF110, have been 
      isolated as DNA- and RNA-binding proteins together with the less-studied protein 
      NF45. These complexes have been implicated in gene regulation, but little is 
      known about their cellular roles and whether they are redundant or functionally 
      distinct. We show that heterodimeric core complexes, NF90-NF45 and NF110-NF45, 
      exist within larger complexes that are more labile and contain multiple NF90/110 
      isoforms and additional proteins. Depletion of the NF45 subunit by RNA 
      interference is accompanied by a dramatic decrease in the levels of NF90 and 
      NF110. Reciprocally, depletion of NF90 but not of NF110 greatly reduces the level 
      of NF45. Coregulation of NF90 and NF45 is a posttranscriptional phenomenon, 
      resulting from protein destabilization in the absence of partners. Depletion of 
      NF90-NF45 complexes retards cell growth by inhibition of DNA synthesis. Giant 
      multinucleated cells containing nuclei attached by constrictions accumulate when 
      either NF45 or NF90, but not NF110, is depleted. This study identified NF45 as an 
      unstable regulatory subunit of NF90-NF45 complexes and uncovered their critical 
      role in normal cell division. Furthermore, the study revealed that NF90 is 
      functionally distinct from NF110 and is more important for cell growth.
FAU - Guan, Deyu
AU  - Guan D
AD  - Departments of Biochemistry and Molecular Biology, New Jersey Medical School, 
      University of Medicine and Dentistry of New Jersey, Newark, New Jersey 
      07103-1709, USA.
FAU - Altan-Bonnet, Nihal
AU  - Altan-Bonnet N
FAU - Parrott, Andrew M
AU  - Parrott AM
FAU - Arrigo, Cindy J
AU  - Arrigo CJ
FAU - Li, Quan
AU  - Li Q
FAU - Khaleduzzaman, Mohammed
AU  - Khaleduzzaman M
FAU - Li, Hong
AU  - Li H
FAU - Lee, Chee-Gun
AU  - Lee CG
FAU - Pe'ery, Tsafi
AU  - Pe'ery T
FAU - Mathews, Michael B
AU  - Mathews MB
LA  - eng
GR  - F32 GM069139/GM/NIGMS NIH HHS/United States
GR  - R01 AI034552/AI/NIAID NIH HHS/United States
GR  - GM069139/GM/NIGMS NIH HHS/United States
GR  - AI34552/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080505
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (ILF2 protein, human)
RN  - 0 (ILF3 protein, human)
RN  - 0 (Nuclear Factor 45 Protein)
RN  - 0 (Nuclear Factor 90 Proteins)
SB  - IM
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Giant Cells/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - *Mitosis
MH  - Nuclear Factor 45 Protein/genetics/*metabolism
MH  - Nuclear Factor 90 Proteins/genetics/*metabolism
MH  - Protein Processing, Post-Translational
MH  - RNA Interference
PMC - PMC2447130
EDAT- 2008/05/07 09:00
MHDA- 2008/07/17 09:00
PMCR- 2008/11/01
CRDT- 2008/05/07 09:00
PHST- 2008/05/07 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2008/05/07 09:00 [entrez]
PHST- 2008/11/01 00:00 [pmc-release]
AID - MCB.00120-08 [pii]
AID - 0120-08 [pii]
AID - 10.1128/MCB.00120-08 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2008 Jul;28(14):4629-41. doi: 10.1128/MCB.00120-08. Epub 2008 May 
      5.