PMID- 1845808
OWN - NLM
STAT- MEDLINE
DCOM- 19910125
LR  - 20190510
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 163
IP  - 1
DP  - 1991 Jan
TI  - Electrophoretic analysis of human herpesvirus 6 polypeptides immunoprecipitated 
      from infected cells with human sera.
PG  - 29-34
AB  - Proteins of human herpesvirus 6 (HHV-6) eliciting human antibody responses were 
      examined in serum from healthy adults and patients with AIDS, chronic fatigue 
      syndrome, Hodgkin's disease, and Sjogren's syndrome. HHV-6 IgG antibody titers 
      measured by immunofluorescence (IF) ranged from 1:10 to 1:1280. Lysates of 
      HHV-6-infected and uninfected cells labeled with [35S]methionine, 
      [3H]glucosamine, and 125I were immunoprecipitated with sera and analyzed 
      electophoretically. Sera with IF titers greater than or equal to 1:20 
      immunoprecipitated greater than 20 [35S]methionine-labeled HHV-6 polypeptides of 
      approximately 26-180 kDa. At least 10 HHV-6 glycoproteins and 8 HHV-6 
      polypeptides associated with the surfaces of infected cells were recognized by 
      human sera. The approximate molecular masses of glycoproteins immunoprecipitated 
      by human sera were similar to those immunoprecipitated by monoclonal antibodies. 
      The labeling intensity of HHV-6 protein bands increased with increasing IF titer, 
      and the effect was most prominent for HHV-6 glycopolypeptides. No reactivities 
      with specific HHV-6 polypeptide(s) were characteristic of a given patient group. 
      These findings suggest that HHV-6 glycoproteins are good targets for human 
      antibody responses.
FAU - Balachandran, N
AU  - Balachandran N
AD  - Department of Microbiology, Molecular Genetics, and Immunology, University of 
      Kansas Medical Center, Kansas City 66103.
FAU - Tirawatnapong, S
AU  - Tirawatnapong S
FAU - Pfeiffer, B
AU  - Pfeiffer B
FAU - Ablashi, D V
AU  - Ablashi DV
FAU - Salahuddin, S Z
AU  - Salahuddin SZ
LA  - eng
GR  - AI-24224/AI/NIAID NIH HHS/United States
GR  - RR-05373/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Immune Sera)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Adult
MH  - Antibodies, Viral/*immunology
MH  - Antigens, Viral/analysis
MH  - Cell Line
MH  - Densitometry
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Immune Sera/immunology
MH  - Peptides/analysis
MH  - Radioimmunoprecipitation Assay
MH  - Viral Proteins/*analysis/immunology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1093/infdis/163.1.29 [doi]
PST - ppublish
SO  - J Infect Dis. 1991 Jan;163(1):29-34. doi: 10.1093/infdis/163.1.29.