PMID- 18460022
OWN - NLM
STAT- MEDLINE
DCOM- 20080617
LR  - 20171116
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Linking)
VI  - 99
IP  - 7
DP  - 2008 Jul
TI  - The diagnostic value of SYT-SSX detected by reverse transcriptase-polymerase 
      chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH) for 
      synovial sarcoma: a review and prospective study of 255 cases.
PG  - 1355-61
LID - 10.1111/j.1349-7006.2008.00830.x [doi]
AB  - This study aimed to evaluate the diagnostic value of SYT-SSX detected by reverse 
      transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ 
      hybridization (FISH) for synovial sarcoma (SS) in known and potential cases. 
      SYT-SSX was analyzed in formalin-fixed, paraffin-embedded tissues of 62 known SS, 
      60 non-SS and 133 potential SS by RT-PCR and FISH. FISH was mainly performed on a 
      tissue microarray with some modifications. SYT-SSX was detected in 94.7% (54/57) 
      of known SS and 70.5% (86/122) of potential SS by RT-PCR and in 96.7% (58/60) of 
      known SS and 78.1% (100/128) of potential SS by FISH. Moreover, SYT-SSX was 
      negative in 100% (58/58) of non-SS by RT-PCR and in 100% (59/59) of non-SS by 
      FISH. Accordingly, SYT-SSX was detected in 106 potential SS by RT-PCR or FISH, 
      including 80 cases manifested by both methods, 20 specimens verified only by FISH 
      and 6 samples confirmed only by RT-PCR. Clinical findings and 
      immunohistochemistry data were analyzed in potential SS with final molecular 
      diagnosis. The positive ratio of cytokeratin (CK) and epithelial membrane antigen 
      (EMA) in finally diagnosed SS was 51.9% (55/106) and 61.3% (65/106), 
      respectively. Except EMA, clinical parameters (age, sex, tumor size, tumor sites) 
      and other immunohistochemistry indexes (CK, S-100, neurone specific enolase 
      (NSE), CD99, myoglobin, smooth muscle actin (SMA), cluster of differentiation 
      (CD) 68 and mesothelial cell) had no significant difference between finally 
      diagnosed SS and non-SS. It is indicated that the efficiency of FISH is 
      comparable to or even higher than that of RT-PCR for SYT-SSX detection. The 
      detection of SYT-SSX by RT-PCR or FISH is very useful for the final diagnosis of 
      potential synovial sarcomas.
FAU - Sun, Baocun
AU  - Sun B
AD  - Department of Pathology, Cancer Institute & Hospital, Tianjin Medical University, 
      Tianjin 300060, China.
FAU - Sun, Yan
AU  - Sun Y
FAU - Wang, Jian
AU  - Wang J
FAU - Zhao, Xiulan
AU  - Zhao X
FAU - Zhang, Shiwu
AU  - Zhang S
FAU - Liu, Yanxue
AU  - Liu Y
FAU - Li, Xiaoqing
AU  - Li X
FAU - Feng, Yumei
AU  - Feng Y
FAU - Zhou, Hongyuan
AU  - Zhou H
FAU - Hao, Xishan
AU  - Hao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080502
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - 0 (12E7 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (CD99 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (S100 Proteins)
RN  - 0 (SYT-SSX fusion protein)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - 12E7 Antigen
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Cell Adhesion Molecules/analysis
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Phosphopyruvate Hydratase/analysis
MH  - Prospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - S100 Proteins/analysis
MH  - Sarcoma, Synovial/*diagnosis/genetics
MH  - Tissue Array Analysis
EDAT- 2008/05/08 09:00
MHDA- 2008/06/18 09:00
CRDT- 2008/05/08 09:00
PHST- 2008/05/08 09:00 [pubmed]
PHST- 2008/06/18 09:00 [medline]
PHST- 2008/05/08 09:00 [entrez]
AID - CAS830 [pii]
AID - 10.1111/j.1349-7006.2008.00830.x [doi]
PST - ppublish
SO  - Cancer Sci. 2008 Jul;99(7):1355-61. doi: 10.1111/j.1349-7006.2008.00830.x. Epub 
      2008 May 2.