PMID- 18461481
OWN - NLM
STAT- MEDLINE
DCOM- 20080813
LR  - 20211020
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 16
IP  - 3
DP  - 2008
TI  - Changes in chromosome organization during PHA-activation of resting human 
      lymphocytes measured by cryo-FISH.
PG  - 413-26
LID - 10.1007/s10577-008-1230-x [doi]
AB  - During interphase, chromosomes are arranged into territories within a highly 
      organized nuclear space containing several compartments. It is becoming clear 
      that this complex nuclear arrangement is important for gene regulation and 
      therefore expression. The study of chromosome organization in interphase requires 
      high-resolution imaging methods that at the same time allow for flexible 
      labelling strategies and preserve nuclear structure. Tokuyasu cryosections of 
      cells or tissues provide a simple, high-resolution platform for performing 
      immunolabelling and fluorescence in situ hybridization (FISH) on well-preserved 
      samples. Here we show how FISH performed on thin cryosections (cryo-FISH) can be 
      used for the study of chromosome organization at high resolution and in a 
      quantitative manner. We have measured chromosome intermingling, volume and radial 
      position, in resting and activated human lymphocytes, and observed 
      chromosome-specific differences between the two cellular states. These 
      differences are in part related to the nuclear expansion that occurs during 
      activation, but are also likely to be tied to their different transcriptional 
      profiles. Extrapolation of our dataset to the whole genome suggests that 
      activated cells contain a lower amount of chromatin involved in intermingling 
      than resting cells.
FAU - Branco, Miguel R
AU  - Branco MR
AD  - MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, 
      Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK.
FAU - Branco, Tiago
AU  - Branco T
FAU - Ramirez, Francisco
AU  - Ramirez F
FAU - Pombo, Ana
AU  - Pombo A
LA  - eng
GR  - MC_U120061476/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Phytohemagglutinins)
SB  - IM
MH  - Chromosomes, Human/drug effects/*genetics/*ultrastructure
MH  - Cryoelectron Microscopy/*methods
MH  - Cryoultramicrotomy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - In Vitro Techniques
MH  - Lymphocyte Activation/drug effects/genetics
MH  - Lymphocytes/drug effects/ultrastructure
MH  - Microscopy, Confocal/methods
MH  - Phytohemagglutinins/pharmacology
EDAT- 2008/05/08 09:00
MHDA- 2008/08/14 09:00
CRDT- 2008/05/08 09:00
PHST- 2008/05/08 09:00 [pubmed]
PHST- 2008/08/14 09:00 [medline]
PHST- 2008/05/08 09:00 [entrez]
AID - 10.1007/s10577-008-1230-x [doi]
PST - ppublish
SO  - Chromosome Res. 2008;16(3):413-26. doi: 10.1007/s10577-008-1230-x.