PMID- 18462257
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20080827
IS  - 1600-0609 (Electronic)
IS  - 0902-4441 (Linking)
VI  - 81
IP  - 2
DP  - 2008 Aug
TI  - Biallelic deletion 13q14.3 in patients with chronic lymphocytic leukemia: 
      cytogenetic, FISH and clinical studies.
PG  - 94-9
LID - 10.1111/j.1600-0609.2008.01086.x [doi]
AB  - BACKGROUND AND OBJECTIVE: Monoallelic deletion of 13q14.3 (13q14x1) is the most 
      common abnormality in chronic lymphocytic leukemia (CLL). As a sole alteration, 
      it predicts a favorable outcome. Biallelic 13q14.3 (13q14x2) deletion or 
      concomitant 13q14x1/13q14x2 has been scarcely evaluated in the literature. We 
      present the clinical, cytogenetic and fluorescence in situ hybridization (FISH) 
      analysis of six CLL patients with normal karyotypes and 13q14x2 and their 
      comparison to cases with 13q14x1 as a single abnormality. PATIENTS AND METHODS: A 
      total of 103 CLL patients were studied. Cytogenetic and FISH analysis were 
      performed on stimulated peripheral blood lymphocytes. Specific fluorescence DNA 
      probes for CLL were used. RESULTS: Six out of 103 (5.8%) patients showed normal 
      karyotypes and 13q14x2. It was observed as a single alteration in one patient and 
      combined with 13q14x1 in five cases. Biallelic clones were larger than 
      monoallelic ones in 3/5 patients (60%). The comparison of clinical and 
      hematological data between 13q14x1 and 13q14x2 groups showed progression of the 
      disease in all 13q14x2 patients respect to 12/32 (37.5%) cases with 13q14x1 (P = 
      0.008), significant differences in the distribution by Rai stage (P = 0.042) and 
      a tendency of a higher lactate dehydrogenase level in 13q14x2 patients (P = 
      0.054). Treatment free survival for 13q14x2 group was 28.5 months, shorter than 
      those observed in patients with 13q14x1 alone (49 months). CONCLUSIONS: Our data 
      would suggest that 13q14x2 could represent a more aggressive FISH anomaly than 
      13q14x1 alone, probably as a consequence of clonal evolution and/or due to the 
      complete inactivation of this critical region by mean of more complex mechanisms.
FAU - Chena, Christian
AU  - Chena C
AD  - Departamento de Genetica, Instituto de Investigaciones Hematologicas 'Mariano 
      R.Castex', Academia Nacional de Medicina.
FAU - Avalos, Julio Sanchez
AU  - Avalos JS
FAU - Bezares, Raimundo F
AU  - Bezares RF
FAU - Arrossagaray, Guillermo
AU  - Arrossagaray G
FAU - Turdo, Karina
AU  - Turdo K
FAU - Bistmans, Alicia
AU  - Bistmans A
FAU - Slavutsky, Irma
AU  - Slavutsky I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080506
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
SB  - IM
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 13
MH  - Cytogenetic Analysis
MH  - Disease Progression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/mortality/pathology
MH  - Lymphocytes
MH  - Survival Rate
EDAT- 2008/05/09 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/05/09 09:00
PHST- 2008/05/09 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/05/09 09:00 [entrez]
AID - EJH1086 [pii]
AID - 10.1111/j.1600-0609.2008.01086.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2008 Aug;81(2):94-9. doi: 10.1111/j.1600-0609.2008.01086.x. Epub 
      2008 May 6.