PMID- 18463589
OWN - NLM
STAT- MEDLINE
DCOM- 20080718
LR  - 20240109
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 13
IP  - 4
DP  - 2008 Apr 17
TI  - Development of an enzyme-linked immuno-sorbent assay (ELISA) method for 
      carbofuran residues.
PG  - 871-81
AB  - The haptens 
      4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]butanoic acid 
      (BFNB) and 
      6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)-carbonylamino]hexanoic acid 
      (BFNH) were synthesized and then used to develop a rapid,specific and sensitive 
      ELISA method to determine residues of the pesticide carbofuran in a variety of 
      matrices. A hybridoma cell line (5D3) producing anti-carbofuran monoclonal 
      antibodies (MAbs) was also established. Based on the MAbs in combination with the 
      heterologous hapten BFNH coupled to either horseradish peroxidase (HRP) or 
      ovalbumin(OVA), four ELISAs (formats I-IV) for the quantification of carbofuran 
      were developed and compared. Among them, the optimized format II (the 
      conjugate-coated direct competitive ELISA) showed the best characteristics, with 
      an IC50 value of 18.49 ng/mL, a limit of detection of 0.11 ng/mL and the shortest 
      assay time (1 h). This ELISA method was then applied to the determinations of 
      carbofuran in environmental water, soil and food samples. The relative standard 
      deviations (R.S.D.s) ranged from 1.8% to 21.3% and the mean recoveries were 
      104.6%, 108.3%, 106.3% and 100.1% for water, soil, lettuce and cabbage, 
      respectively. Thus, the ELISA method of format II exhibited the potential to 
      develop commercial ELISA kits for a rapid detection of carbofuran for human 
      health and environmental safety.
FAU - Yang, Jinyi
AU  - Yang J
AD  - College of Food Science, South China Agricultural University, Guangzhou 510642, 
      PR China. yjy361@163.com
FAU - Wang, Hong
AU  - Wang H
FAU - Jiang, Yueming
AU  - Jiang Y
FAU - Sun, Yuanming
AU  - Sun Y
FAU - Pan, Ke
AU  - Pan K
FAU - Lei, Hongtao
AU  - Lei H
FAU - Wu, Qing
AU  - Wu Q
FAU - Shen, Yudong
AU  - Shen Y
FAU - Xiao, Zhili
AU  - Xiao Z
FAU - Xu, Zhenlin
AU  - Xu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080417
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 0 (Haptens)
RN  - 0 (Pesticide Residues)
RN  - 0 (Soil)
RN  - 059QF0KO0R (Water)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - SKF77S6Y67 (Carbofuran)
SB  - IM
MH  - Antibodies, Monoclonal/biosynthesis/isolation & purification
MH  - Antigens/chemistry
MH  - Brassica/chemistry
MH  - Carbofuran/*analysis
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Haptens/chemistry
MH  - Horseradish Peroxidase/metabolism
MH  - Lactuca/chemistry
MH  - Pesticide Residues/*analysis
MH  - Soil/analysis
MH  - Water/chemistry
PMC - PMC6244836
EDAT- 2008/05/09 09:00
MHDA- 2008/07/19 09:00
PMCR- 2008/04/17
CRDT- 2008/05/09 09:00
PHST- 2007/03/27 00:00 [received]
PHST- 2008/04/14 00:00 [revised]
PHST- 2008/04/14 00:00 [accepted]
PHST- 2008/05/09 09:00 [pubmed]
PHST- 2008/07/19 09:00 [medline]
PHST- 2008/05/09 09:00 [entrez]
PHST- 2008/04/17 00:00 [pmc-release]
AID - 13040871 [pii]
AID - molecules-13-00871 [pii]
AID - 10.3390/molecules13040871 [doi]
PST - epublish
SO  - Molecules. 2008 Apr 17;13(4):871-81. doi: 10.3390/molecules13040871.