PMID- 18464840 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20121002 LR - 20190917 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 40 IP - 4 DP - 1997 Aug TI - FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor. PG - 475-8 AB - Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads of chromosomes of a sorghum plant containing an extra copy of one arm of the sorghum chromosome arbitrarily designated with the letter D. Therefore, the sequence cloned in this BAC must reside in the arm of chromosome D represented by this monotelosome. This demonstrates a novel procedure for physically mapping cloned genes or other single-copy sequences by FISH, sh2 in this case, by using BACs containing their complementary sequences. The results reported herein suggest homology, at least in part, between one arm of chromosome D in sorghum and the long arm of chromosome 3 in maize. FAU - Gomez, M I AU - Gomez MI FAU - Islam-Faridi, M N AU - Islam-Faridi MN FAU - Woo, S S AU - Woo SS FAU - Czeschin, D Jr AU - Czeschin D Jr FAU - Zwick, M S AU - Zwick MS FAU - Stelly, D M AU - Stelly DM FAU - Price, H J AU - Price HJ FAU - Schertz, K F AU - Schertz KF FAU - Wing, R A AU - Wing RA LA - eng PT - Journal Article PL - Canada TA - Genome JT - Genome JID - 8704544 EDAT- 1997/08/01 00:00 MHDA- 1997/08/01 00:01 CRDT- 1997/08/01 00:00 PHST- 1997/08/01 00:00 [pubmed] PHST- 1997/08/01 00:01 [medline] PHST- 1997/08/01 00:00 [entrez] AID - g97-063 [pii] AID - 10.1139/g97-063 [doi] PST - ppublish SO - Genome. 1997 Aug;40(4):475-8. doi: 10.1139/g97-063.