PMID- 18464864 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20121002 LR - 20190917 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 40 IP - 5 DP - 1997 Oct TI - Structure of the rye midget chromosome analyzed by FISH and C-banding. PG - 782-4 AB - The diminutive "midget" chromosome derived from rye (Secale cereale) was analyzed by C-banding and fluorescence in situ hybridization (FISH) using DNA probe pSau3A9 that is located in the centromeres of cereal chromosomes. FISH signals were detected at one end and overlapped one of the two telomeres of the midget, indicating that the midget is a telocentric chromosome. The FISH and C-banding results show that the centromere of the midget chromosome is smaller than those of normal wheat and rye chromosomes. These results indicate that one of the breakpoints occurred in the middle of the centromere of rye chromosome 1R during generation of the midget. FAU - Jackson, S A AU - Jackson SA FAU - Jiang, J AU - Jiang J FAU - Friebe, B AU - Friebe B FAU - Gill, B S AU - Gill BS LA - eng PT - Journal Article PL - Canada TA - Genome JT - Genome JID - 8704544 EDAT- 2008/05/10 09:00 MHDA- 2008/05/10 09:01 CRDT- 2008/05/10 09:00 PHST- 2008/05/10 09:00 [pubmed] PHST- 2008/05/10 09:01 [medline] PHST- 2008/05/10 09:00 [entrez] AID - g97-801 [pii] AID - 10.1139/g97-801 [doi] PST - ppublish SO - Genome. 1997 Oct;40(5):782-4. doi: 10.1139/g97-801.