PMID- 18464921
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20211020
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2008
DP  - 2008
TI  - Mycoplasma genitalium lipoproteins induce human monocytic cell expression of 
      proinflammatory cytokines and apoptosis by activating nuclear factor kappaB.
PG  - 195427
LID - 10.1155/2008/195427 [doi]
LID - 195427
AB  - This study was designed to investigate the molecular mechanisms responsible for 
      the induction of proinflammatory cytokines gene expression and apoptosis in human 
      monocytic cell line THP-1 stimulated by lipoproteins (LPs) prepared from 
      Mycoplasma genitalium. Cultured cells were stimulated with M. genitalium LP to 
      analyze the production of proinflammatory cytokines and expression of their mRNA 
      by ELISA and RT-PCR, respectively. Cell apoptosis was also detected by Annexin 
      V-FITC-propidium iodide (PI) staining and acridine orange (AO)-ethidium bromide 
      (EB) staining. The DNA-binding activity of nuclear factor-kappaB (NF-kappaB) was 
      assessed by electrophoretic mobility shift assay (EMSA). Results showed that LP 
      stimulated THP-1 cells to produce tumor necrosis factor-alpha (TNF-alpha), 
      interleukin-1beta (IL-1beta), and IL-6 in a dose-dependent manner. The mRNA 
      levels were also upregulated in response to LP stimulation. LPs were also found 
      to increase the DNA-binding activity of NF-kappaB, a possible mechanism for the 
      induction of cytokine mRNA expression and the cell apoptosis. These effects were 
      abrogated by PDTC, an inhibitor of NF-kappaB. Our results indicate that M. 
      genitalium-derived LP may be an important etiological factor of certain diseases 
      due to the ability of LP to produce proinflammatory cytokines and induction of 
      apoptosis, which is probably mediated through the activation of NF-kappaB.
FAU - Wu, Yimou
AU  - Wu Y
AD  - Pathogenic Biology Institute, University of South China, 421001 Hengyang, China. 
      yimouwu@sina.com
FAU - Qiu, Hong
AU  - Qiu H
FAU - Zeng, Yanhua
AU  - Zeng Y
FAU - You, Xiaoxing
AU  - You X
FAU - Deng, Zhongliang
AU  - Deng Z
FAU - Yu, Minjun
AU  - Yu M
FAU - Zhu, Cuiming
AU  - Zhu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Annexin A5)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Annexin A5/analysis
MH  - Apoptosis/*drug effects
MH  - Bacterial Proteins/*pharmacology
MH  - Cell Line
MH  - Cytokines/*biosynthesis/genetics
MH  - Humans
MH  - Interleukin-1beta/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Lipoproteins/*pharmacology
MH  - Mycoplasma genitalium/*chemistry
MH  - NF-kappa B/*metabolism
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/biosynthesis
PMC - PMC2366083
EDAT- 2008/05/10 09:00
MHDA- 2008/06/25 09:00
PMCR- 2008/04/23
CRDT- 2008/05/10 09:00
PHST- 2007/08/29 00:00 [received]
PHST- 2007/11/21 00:00 [revised]
PHST- 2008/01/23 00:00 [accepted]
PHST- 2008/05/10 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2008/05/10 09:00 [entrez]
PHST- 2008/04/23 00:00 [pmc-release]
AID - 10.1155/2008/195427 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2008;2008:195427. doi: 10.1155/2008/195427.