PMID- 18467834
OWN - NLM
STAT- MEDLINE
DCOM- 20080529
LR  - 20080509
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 120
IP  - 1-2
DP  - 2008
TI  - Three autosomal chromosome translocations associated with repeated early 
      embryonic loss (REEL) in the domestic horse (Equus caballus).
PG  - 117-22
LID - 10.1159/000118749 [doi]
AB  - Repeated early embryonic loss (REEL) represents a considerable economic loss to 
      the horse industry. Mares that experience REEL may be overlooked as potential 
      carriers of a chromosome abnormality. Here we report three different autosomal 
      translocations in Thoroughbred mares presented for chromosome analysis because of 
      REEL. The karyotypes were 64,XX,t(1;21), 64,XX,t(16;22), and 64,XX,t(4;13), 
      respectively. In order to confirm the chromosomes involved in the translocations, 
      to map the breakpoints, and to determine if the translocations were reciprocal, 
      genes surrounding the breakpoints were identified using existing maps and from 
      the newly assembled horse genome sequence. Bacterial artificial chromosomes 
      containing the genes of interest were identified and mapped to the translocation 
      chromosomes by fluorescence in situ hybridization (FISH). FISH confirmed that the 
      t(16;22) and t(4;13) translocations were reciprocal, while the t(1;21) was not. 
      The breakpoints on horse chromosomes 1 and 16 appear to be the same or near 
      breakpoints previously identified in translocations. These breakpoints are at the 
      fusion boundary of human chromosomes 10 and 15 on horse chromosome 1 and at human 
      chromosome 3p and 3q on horse chromosome 16. These sites may represent ancient 
      breakpoints reused during equid evolution. Overall, chromosome abnormalities may 
      have a greater influence on mare fertility than previously known. Thus, it is 
      important to karyotype subfertile mares exhibiting REEL.
CI  - Copyright 2008 S. Karger AG, Basel.
FAU - Lear, T L
AU  - Lear TL
AD  - Maxwell H. Gluck Equine Research Center, Veterinary Science Department, 
      University of Kentucky, Lexington, KY, USA. equigene@uky.edu
FAU - Lundquist, J
AU  - Lundquist J
FAU - Zent, W W
AU  - Zent WW
FAU - Fishback, W D Jr
AU  - Fishback WD Jr
FAU - Clark, A
AU  - Clark A
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080430
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Abortion, Habitual/genetics/*veterinary
MH  - Animals
MH  - Chromosome Banding/veterinary
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Female
MH  - Horse Diseases/*genetics
MH  - Horses/*genetics
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Male
MH  - Pregnancy
MH  - *Translocation, Genetic
EDAT- 2008/05/10 09:00
MHDA- 2008/05/30 09:00
CRDT- 2008/05/10 09:00
PHST- 2007/12/05 00:00 [accepted]
PHST- 2008/05/10 09:00 [pubmed]
PHST- 2008/05/30 09:00 [medline]
PHST- 2008/05/10 09:00 [entrez]
AID - 000118749 [pii]
AID - 10.1159/000118749 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2008;120(1-2):117-22. doi: 10.1159/000118749. Epub 2008 Apr 
      30.