PMID- 18468248
OWN - NLM
STAT- MEDLINE
DCOM- 20081003
LR  - 20190608
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 17
IP  - 1-2
DP  - 2008
TI  - Cloning and characterization of liver progenitor cells from the scattered cell 
      clusters in primary culture of porcine livers.
PG  - 179-86
AB  - The scattered cell clusters that can differentiate into hepatocytes or biliary 
      epithelial cells have been isolated from primary cultures of adult porcine 
      livers. We have generated 11 clonal cell lines from this system and identified 
      liver progenitor cells (LPCs) among the clonal lines. These clonal lines 
      expressed c-kit, HNF-1, HNF-6, and/or CK19 mRNA. An immunocytochemical study of 
      the clonal lines indicated that clonal line CL-11 expressed liver epithelial cell 
      markers CK14, vimentin, CK18, and BD-1. The expression of albumin and 
      alpha1-antitrypsin (alpha1-AT) mRNA was only upregulated in CL-11 among the 
      clonal lines when they were grown as aggregates. Under these conditions, CL-11 
      also exhibited ammonia metabolic activity and several indicators that suggest 
      hepatocytic differentiation, including the upregulation of liver-specific genes 
      such as dipeptidyl peptidase IV, CYP1A1, and CYP3A4 mRNA, and the downregulation 
      of biliary cell markers such as gamma-glutamyltrans-peptidase (GGT), CK19, and 
      HNF6 mRNA. After culturing CL-11 in Matrigel, the expression of GGT and HNF6 mRNA 
      was upregulated. These results indicate that CL-11 has dual potential: the 
      ability to differentiate as hepatocytes or as bile duct cells. The isolation of 
      scattered cells could provide a simple method to generate LPC lines from adult 
      livers.
FAU - Tokiwa, Takayoshi
AU  - Tokiwa T
AD  - Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, 
      Tokyo 140-0001, Japan. t.tokiwa@kcmi.or.jp
FAU - Yamazaki, Taisuke
AU  - Yamazaki T
FAU - Ono, Masashi
AU  - Ono M
FAU - Enosawa, Shin
AU  - Enosawa S
FAU - Tsukiyama, Takashi
AU  - Tsukiyama T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Biomarkers)
RN  - 0 (Hepatocyte Nuclear Factor 4)
RN  - 0 (Hepatocyte Nuclear Factor 6)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - EC 1.14.14.55 (CYP3A4 protein, human)
RN  - EC 3.4.14.5 (DPP4 protein, human)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
SB  - IM
MH  - Animals
MH  - Bile Ducts/cytology
MH  - Biomarkers/analysis
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Clone Cells
MH  - Cytochrome P-450 CYP3A/biosynthesis
MH  - Cytochrome P-450 Enzyme System/biosynthesis
MH  - Dipeptidyl Peptidase 4/biosynthesis
MH  - Epithelial Cells/cytology/physiology
MH  - Hepatocyte Nuclear Factor 4/biosynthesis
MH  - Hepatocyte Nuclear Factor 6/biosynthesis
MH  - *Hepatocytes/cytology/physiology
MH  - *Stem Cells/cytology/physiology
MH  - Sus scrofa
EDAT- 2008/05/13 09:00
MHDA- 2008/10/04 09:00
CRDT- 2008/05/13 09:00
PHST- 2008/05/13 09:00 [pubmed]
PHST- 2008/10/04 09:00 [medline]
PHST- 2008/05/13 09:00 [entrez]
AID - 10.3727/000000008783907080 [doi]
PST - ppublish
SO  - Cell Transplant. 2008;17(1-2):179-86. doi: 10.3727/000000008783907080.