PMID- 18468651
OWN - NLM
STAT- MEDLINE
DCOM- 20080918
LR  - 20191210
IS  - 0041-0101 (Print)
IS  - 0041-0101 (Linking)
VI  - 51
IP  - 8
DP  - 2008 Jun 15
TI  - Pouterin, a novel potential cytotoxic lectin-like protein with apoptosis-inducing 
      activity in tumorigenic mammalian cells.
PG  - 1321-30
LID - 10.1016/j.toxicon.2008.03.007 [doi]
AB  - In this study, the cytotoxicity of pouterin in tumorigenic and non-tumorigenic 
      mammalian cell lines was investigated. We found that HeLa, Hep-2 and HT-29 tumor 
      cells were highly sensitive to pouterin cytotoxicity in a dose-dependent manner, 
      whereas non-tumorigenic Vero cells and human lymphocytes were relatively 
      resistant to the protein. Among the tumor cell lines, HeLa cells showed the 
      highest susceptibility to pouterin cytotoxicity, exhibiting a time-dependent 
      increase in LDH leakage and an IC(50) value of 5mug/mL. Morphological alterations 
      such as rounding, cell shrinkage and chromatin condensation, consistent with 
      apoptotic cell death were observed. Apoptosis induction was demonstrated by DNA 
      fragmentation as detected by terminal dUTP nick-end labeling (TUNEL). 
      Furthermore, HeLa cells incubated with pouterin showed disruption of the actin 
      cytoskeleton. Western blot analysis revealed that pouterin caused increased 
      expression of p21, thus indicating cell cycle arrest. Subsequent studies provided 
      evidence that apoptosis may be partially explained in the activation of the tumor 
      necrosis factor receptor 1 (TNFR1) signaling. Interestingly, a time-dependent 
      decrease of the expression of p65 nuclear factor kappa B (NFkappaB) subunit, 
      concomitant with a downregulation of the inhibitor of apoptosis protein 1 (IAP1) 
      was observed, suggesting that TNFR-mediated apoptosis is the predominant pathway 
      induced by pouterin in HeLa cells.
FAU - Boleti, Ana Paula de A
AU  - Boleti AP
AD  - Departamento de Bioquimica/IB, Universidade Estadual de Campinas, Campinas, SP, 
      Brazil.
FAU - Ventura, Claudio A
AU  - Ventura CA
FAU - Justo, Giselle Z
AU  - Justo GZ
FAU - Silva, Rodrigo A
AU  - Silva RA
FAU - de Sousa, Ana Carolina T
AU  - de Sousa AC
FAU - Ferreira, Carmen V
AU  - Ferreira CV
FAU - Yano, Tomomasa
AU  - Yano T
FAU - Macedo, Maria Ligia R
AU  - Macedo ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080318
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Cytotoxins)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Lectins)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
SB  - IM
MH  - Actin Cytoskeleton/drug effects/ultrastructure
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*toxicity
MH  - *Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Nucleus/drug effects/ultrastructure
MH  - Chlorocebus aethiops
MH  - Cytotoxins/*toxicity
MH  - DNA Fragmentation
MH  - HeLa Cells
MH  - Humans
MH  - Lymphocytes/drug effects/ultrastructure
MH  - NF-kappa B/genetics/metabolism
MH  - Plant Lectins/*toxicity
MH  - Pouteria/chemistry
MH  - Receptors, Tumor Necrosis Factor, Type I/genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - Up-Regulation
MH  - Vero Cells
EDAT- 2008/05/13 09:00
MHDA- 2008/09/19 09:00
CRDT- 2008/05/13 09:00
PHST- 2007/07/18 00:00 [received]
PHST- 2008/02/28 00:00 [revised]
PHST- 2008/03/03 00:00 [accepted]
PHST- 2008/05/13 09:00 [pubmed]
PHST- 2008/09/19 09:00 [medline]
PHST- 2008/05/13 09:00 [entrez]
AID - S0041-0101(08)00079-2 [pii]
AID - 10.1016/j.toxicon.2008.03.007 [doi]
PST - ppublish
SO  - Toxicon. 2008 Jun 15;51(8):1321-30. doi: 10.1016/j.toxicon.2008.03.007. Epub 2008 
      Mar 18.