PMID- 18470140
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20240109
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 37
IP  - 6
DP  - 1994 Dec
TI  - Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus 
      coccineus by fluorescence in situ hybridization after pepsin pretreatment.
PG  - 1018-21
AB  - This is the first report of fluorescence in situ hybridization (FISH) on plant 
      polytene chromosomes. Different protease pretreatments have been tested to 
      improve fluorescence in situ hybridization FISH on polytene chromosomes of a 
      plant, Phaseolus coccineus, with the aim to enable the detection of low-copy 
      genes. The structural preservation of the chromosomes and the distinctness of the 
      FISH signals were comparatively analysed with a probe for the ribosomal RNA genes 
      after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a 
      general loosening of chromatin with good conservation of chromosome morphology 
      and an increased number and density of signal points. The six nucleolus 
      organizers exhibited significant differences in condensation. The pretreatment 
      with pepsin enabled the detection of the low-copy genes encoding the seed storage 
      protein phaseolin.
FAU - Nenno, M
AU  - Nenno M
FAU - Schumann, K
AU  - Schumann K
FAU - Nagl, W
AU  - Nagl W
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - g94-144 [pii]
AID - 10.1139/g94-144 [doi]
PST - ppublish
SO  - Genome. 1994 Dec;37(6):1018-21. doi: 10.1139/g94-144.