PMID- 18470304
OWN - NLM
STAT- MEDLINE
DCOM- 20080925
LR  - 20230411
IS  - 0091-6765 (Print)
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Linking)
VI  - 116
IP  - 5
DP  - 2008 May
TI  - The polybrominated diphenyl ether mixture DE-71 is mildly estrogenic.
PG  - 605-11
LID - 10.1289/ehp.10643 [doi]
AB  - BACKGROUND: Polybrominated diphenyl ethers (PBDEs) are widely found in the 
      environment, and they may act as endocrine disruptors. OBJECTIVE: Our goal in 
      this study was to test the PBDE mixture DE-71 for estrogenic activity. METHODS: 
      We used proliferation of cultured breast cancer cells (MCF-7) and trophic effects 
      in the reproductive tracts of ovariectomized mice as estrogen bioassays. DE-71 
      was administered to mice by subcutaneous injection (sc) or oral gavage (po), 
      alone or in combination with estradiol, for 3 or 34 days. Liver weights and 
      cytochrome P450 enzyme activities were also measured. RESULTS: DE-71 increased 
      MCF-7 cell proliferation, and this was prevented by antiestrogen. DE-71 
      cotreatment reduced the effect of estradiol in MCF-7 cells. In the mouse 3-day 
      assay, DE-71 administered alone had no effect on uterine weight, uterine 
      epithelial height (UEH), or vaginal epithelial thickness (VET); however, when 
      DE-71 was administered as a cotreatment, it potentiated estradiol's effect on 
      uterine weight. DE-71 administered sc to BALB/c mice for 34 days slightly 
      increased UEH and VET, and attenuated the estradiol-induced increase in UEH; 
      these effects were not seen in BALB/c mice treated po or in C57BL/6 mice treated 
      sc. DE-71 increased liver weight in BALB/c, C57BL/6, and estrogen receptor-alpha 
      knockout mice. We also found an increase in liver cytochrome P450 1A (CYP1A) and 
      CYP2B activities when DE-71 was administered po, but only CYP2B increased after 
      sc treatment. CONCLUSION: DE-71 behaves as a weak estrogen. In mice, the 
      treatment route and duration determined if DE-71 was estrogenic. BALB/c mice are 
      more susceptible to DE-71 effects in estrogen target tissues than C57BL/6 mice. 
      DE-71 increased liver weight independently of estrogen receptor-alpha.
FAU - Mercado-Feliciano, Minerva
AU  - Mercado-Feliciano M
AD  - Department of Pharmacology and Toxicology, University School of Medicine, 975 W. 
      Walnut St. (IB360), Indianapolis, IN 46202-5121 USA.
FAU - Bigsby, Robert M
AU  - Bigsby RM
LA  - eng
GR  - F31 ES013341/ES/NIEHS NIH HHS/United States
GR  - R21 ES014367/ES/NIEHS NIH HHS/United States
GR  - ES013341/ES/NIEHS NIH HHS/United States
GR  - ES014367/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Estrogen Receptor Modulators)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Phenyl Ethers)
RN  - 0 (Polybrominated Biphenyls)
RN  - 4TI98Z838E (Estradiol)
RN  - 7REL09ZX35 (pentabromodiphenyl ether)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Breast Neoplasms/pathology
MH  - Cell Proliferation/drug effects
MH  - Cytochrome P-450 Enzyme System/analysis
MH  - Endocrine Disruptors
MH  - Estradiol/administration & dosage
MH  - Estrogen Receptor Modulators/*pharmacology
MH  - Female
MH  - Halogenated Diphenyl Ethers
MH  - Liver/cytology/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ovariectomy
MH  - Phenyl Ethers/administration & dosage/*pharmacology
MH  - Polybrominated Biphenyls/administration & dosage/*pharmacology
PMC - PMC2367668
OTO - NOTNLM
OT  - CYP1A
OT  - CYP2B
OT  - DE-71
OT  - MCF-7
OT  - PBDEs
OT  - endocrine disruptors
OT  - estrogens
OT  - mice
OT  - ovariectomized
OT  - polybrominated diphenyl ethers
EDAT- 2008/05/13 09:00
MHDA- 2008/09/26 09:00
PMCR- 2008/05/01
CRDT- 2008/05/13 09:00
PHST- 2007/07/07 00:00 [received]
PHST- 2008/01/25 00:00 [accepted]
PHST- 2008/05/13 09:00 [pubmed]
PHST- 2008/09/26 09:00 [medline]
PHST- 2008/05/13 09:00 [entrez]
PHST- 2008/05/01 00:00 [pmc-release]
AID - ehp0116-000605 [pii]
AID - 10.1289/ehp.10643 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2008 May;116(5):605-11. doi: 10.1289/ehp.10643.