PMID- 18471896
OWN - NLM
STAT- MEDLINE
DCOM- 20080919
LR  - 20080602
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 124
IP  - 1-2
DP  - 2008 Jul 15
TI  - Alloimmunity does not protect from challenge with the feline immunodeficiency 
      virus.
PG  - 152-62
LID - 10.1016/j.vetimm.2008.03.010 [doi]
AB  - Immune responses against polymorphic host molecules incorporated into lentiviral 
      envelopes during cell budding have induced protection against primate 
      immunodeficiency virus infection. Dendritic cells (DCs) express high levels of 
      MHC molecules and are infectable by lentiviruses. Therefore, in this pilot study 
      we addressed the hypothesis that immunization of cats with allogeneic DC would 
      induce immune responses that protect against challenge with the feline 
      immunodeficiency virus. Two groups of 3 cats each received 3 subcutaneous 
      injections of allogeneic or autologous DC, and were then challenged with viruses 
      propagated in the immunizing DC. Infection status and lymphocyte parameters of 
      cats were assessed during 6 weeks after challenge. MHC II antigens were 
      incorporated into viral particles as identified by Western blot; and antibodies 
      reactive with MHC class II antigens were detected in the serum of cats immunized 
      with allogeneic but not autologous DC. After challenge, all cats had proviral DNA 
      in blood leukocytes from 2 weeks post-challenge onward and seroconverted. Cats 
      immunized with allogeneic DC maintained higher total and CD21(+) lymphocyte 
      concentrations, and higher CD4(+)/CD8(+) lymphocyte ratios; however, these 
      differences were not significantly different from cats that received autologous 
      DC immunizations. Plasma viral load was not significantly different between 
      groups of cats (p=0.204). These results suggest that immunization of cats with 
      allogeneic DC does not induce protective immunity against FIV infection.
FAU - Reggeti, F
AU  - Reggeti F
AD  - Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada.
FAU - Bienzle, D
AU  - Bienzle D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080330
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - CD4-CD8 Ratio/veterinary
MH  - Cats
MH  - Cell Survival/immunology
MH  - Dendritic Cells/*immunology
MH  - Feline Acquired Immunodeficiency Syndrome/*immunology/*prevention & 
      control/virology
MH  - Flow Cytometry/veterinary
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Immunization/methods/*veterinary
MH  - Immunoblotting/veterinary
MH  - Immunodeficiency Virus, Feline/*immunology
MH  - Lymphocyte Count/veterinary
MH  - Microscopy, Fluorescence/veterinary
MH  - Pilot Projects
MH  - RNA, Viral/chemistry/genetics
MH  - Random Allocation
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Specific Pathogen-Free Organisms
EDAT- 2008/05/13 09:00
MHDA- 2008/09/20 09:00
CRDT- 2008/05/13 09:00
PHST- 2008/01/07 00:00 [received]
PHST- 2008/02/27 00:00 [revised]
PHST- 2008/03/07 00:00 [accepted]
PHST- 2008/05/13 09:00 [pubmed]
PHST- 2008/09/20 09:00 [medline]
PHST- 2008/05/13 09:00 [entrez]
AID - S0165-2427(08)00107-4 [pii]
AID - 10.1016/j.vetimm.2008.03.010 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2008 Jul 15;124(1-2):152-62. doi: 
      10.1016/j.vetimm.2008.03.010. Epub 2008 Mar 30.