PMID- 18473124
OWN - NLM
STAT- MEDLINE
DCOM- 20090625
LR  - 20211020
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Linking)
VI  - 135
IP  - 2
DP  - 2009 Mar
TI  - Comparative molecular cytogenetic analysis of three Leuciscus species (Pisces, 
      Cyprinidae) using chromosome banding and FISH with rDNA.
PG  - 199-207
LID - 10.1007/s10709-008-9269-3 [doi]
AB  - A comparative molecular cytogenetic analysis was performed on three species of 
      the genus Leuciscus viz. ide L. idus, chub L. cephalus and dace L. leuciscus 
      distributed in Poland, using C-, Ag- and chromomycin A(3) (CMA(3))-stainings and 
      fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. 
      Although the three species examined shared 2n = 50 chromosomes and the largest 
      acrocentric chromosome pair in the complement, they were characterized with 
      karyotypic differences in terms of the number of uni- and biarmed chromosomes and 
      the localization of nucleolar organizer regions (NORs) revealed by Ag-staining 
      and FISH. L. idus and L. cephalus showed the rDNA sites on the long arms of one 
      submetacentric (SM) chromosome pair and on the short arms of one subtelocentric 
      (ST) chromosome pair, respectively. These NORs were CMA(3)-positive, GC-rich and 
      C-positive heterochromatic sites in both species. Such chromosome banding 
      features were also true for four NORs localizing on one of each SM and ST pair in 
      L. leuciscus, but considerable numerical NOR polymorphism became apparent with 
      Ag-staining and FISH due to a different combination of these NOR-bearing SMs and 
      STs in this dace. The present results indicate that the molecular cytogenetic 
      analysis applied herein may become useful to elucidate the karyotype evolution 
      and phylogenetic relationships among the species in the genus Leuciscus and other 
      related groups.
FAU - Boron, Alicja
AU  - Boron A
AD  - Department of Zoology, Faculty of Biology, University of Warmia and Mazury, M. 
      Oczapowskiego 5, Olsztyn, 10-718, Poland. alibo@uwm.edu.pl
FAU - Porycka, Katarzyna
AU  - Porycka K
FAU - Ito, Daisuke
AU  - Ito D
FAU - Abe, Syuiti
AU  - Abe S
FAU - Kirtiklis, Lech
AU  - Kirtiklis L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080513
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - Chromosomes, Plant/*genetics
MH  - Cyprinidae/*genetics
MH  - Cytogenetic Analysis/*methods
MH  - DNA, Plant/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
EDAT- 2008/05/14 09:00
MHDA- 2009/06/26 09:00
CRDT- 2008/05/14 09:00
PHST- 2007/05/30 00:00 [received]
PHST- 2008/04/25 00:00 [accepted]
PHST- 2008/05/14 09:00 [pubmed]
PHST- 2009/06/26 09:00 [medline]
PHST- 2008/05/14 09:00 [entrez]
AID - 10.1007/s10709-008-9269-3 [doi]
PST - ppublish
SO  - Genetica. 2009 Mar;135(2):199-207. doi: 10.1007/s10709-008-9269-3. Epub 2008 May 
      13.