PMID- 1847338
OWN - NLM
STAT- MEDLINE
DCOM- 19910325
LR  - 20190907
IS  - 0014-4800 (Print)
IS  - 0014-4800 (Linking)
VI  - 54
IP  - 1
DP  - 1991 Feb
TI  - Production of monocyte chemoattractant protein-1 by malignant fibrous 
      histiocytoma: relation to the origin of histiocyte-like cells.
PG  - 61-71
AB  - Human malignant fibrous histiocytoma (MFH) comprise both fibroblast-like cells 
      and histiocyte-like cells. We previously showed that the latter are not 
      neoplastic cells, but are infiltrating macrophages. Since migration of blood 
      monocytes into the tumor might be a response to a locally elaborated monocyte 
      chemoattractant, we designed experiments to determine if the fibroblast-like 
      tumor cells produced a chemoattractant for human monocytes. Malignant fibrous 
      histiocytoma from three patients was put into culture. Cells of all three lines 
      had a spindle shape, and showed no reactivity with antibodies against macrophages 
      (MAC387), HLA-DR (LN3), or leukocyte common antigen. Immunohistochemically, they 
      stained with antibody against human monocyte chemoattractant protein-1 (MCP-1). 
      Culture supernatants of the three cell lines had chemotactic activity for 
      monocytes. This activity was due to MCP-1, since it was absorbed by an anti-MCP-1 
      column. The production of MCP-1 by MFH tumor lines was confirmed by 
      immunoprecipitation of metabolically labeled MCP-1. These results suggest that 
      the histiocyte-like cells are the infiltrated macrophages that originate from 
      blood monocytes attracted by tumor-derived MCP-1.
FAU - Takeya, M
AU  - Takeya M
AD  - Second Department of Pathology, Kumamoto University Medical School, Japan.
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Leonard, E J
AU  - Leonard EJ
FAU - Kato, T
AU  - Kato T
FAU - Okabe, H
AU  - Okabe H
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Vimentin)
SB  - IM
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis
MH  - Female
MH  - Histiocytoma, Benign Fibrous/*metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Molecular Weight
MH  - Precipitin Tests
MH  - Tumor Cells, Cultured
MH  - Vimentin/metabolism
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
AID - 0014-4800(91)90044-X [pii]
AID - 10.1016/0014-4800(91)90044-x [doi]
PST - ppublish
SO  - Exp Mol Pathol. 1991 Feb;54(1):61-71. doi: 10.1016/0014-4800(91)90044-x.