PMID- 18474228
OWN - NLM
STAT- MEDLINE
DCOM- 20080620
LR  - 20141120
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 372
IP  - 1
DP  - 2008 Jul 18
TI  - Ceftiofur impairs pro-inflammatory cytokine secretion through the inhibition of 
      the activation of NF-kappaB and MAPK.
PG  - 73-7
LID - 10.1016/j.bbrc.2008.04.170 [doi]
AB  - Ceftiofur is a new broad-spectrum, third-generation cephalosporin antibiotic for 
      veterinary use. Immunopharmacological studies can provide new information on the 
      immunomodulatory activities of some drugs, including their effect on cytokine 
      productions. For this reason, we investigated the effect of ceftiofur on cytokine 
      productions in vitro. We found that ceftiofur can downregulate tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6), 
      but did not affect interleukin-10 (IL-10) production. We further investigated 
      signal transduction mechanisms to determine how ceftiofur affects. RAW 264.7 
      cells were pretreated with 1, 5, or 10 mg/L of ceftiofur 1 h prior to treatment 
      with 1 mg/L of LPS. Thirty minutes later, cells were harvested and mitogen 
      activated protein kinases (MAPKs) activation was measured by Western blot. 
      Alternatively, cells were fixed and nuclear factor-kappaB (NF-kappaB) activation 
      was measured using immunocytochemical analysis. Signal transduction studies 
      showed that ceftiofur significantly inhibited extracellular signal-regulated 
      kinase (ERK), p38, and c-jun NH(2)-terminal kinase (JNK) phosphorylation protein 
      expression. Ceftiofur also inhibited p65-NF-kappaB translocation into the 
      nucleus. Therefore, ceftiofur may inhibit LPS-induced production of inflammatory 
      cytokines by blocking NF-kappaB and MAPKs signaling in RAW264.7 cells.
FAU - Ci, Xinxin
AU  - Ci X
AD  - Department of Veterinary Pharmacology, College of Animal Science and Veterinary 
      Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
FAU - Song, Yu
AU  - Song Y
FAU - Zeng, Fanqin
AU  - Zeng F
FAU - Zhang, Xuemei
AU  - Zhang X
FAU - Li, Hongyu
AU  - Li H
FAU - Wang, Xinrui
AU  - Wang X
FAU - Cui, Junqing
AU  - Cui J
FAU - Deng, Xuming
AU  - Deng X
LA  - eng
PT  - Journal Article
DEP - 20080512
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cephalosporins)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Transcription Factor RelA)
RN  - 83JL932I1C (ceftiofur)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Active Transport, Cell Nucleus/drug effects
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cephalosporins/*pharmacology
MH  - Cytokines/*metabolism
MH  - Lipopolysaccharides/immunology/pharmacology
MH  - Macrophages/*drug effects/immunology
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*antagonists & inhibitors/metabolism
MH  - Signal Transduction/drug effects
MH  - Transcription Factor RelA/agonists/*antagonists & inhibitors
EDAT- 2008/05/14 09:00
MHDA- 2008/06/21 09:00
CRDT- 2008/05/14 09:00
PHST- 2008/04/22 00:00 [received]
PHST- 2008/04/28 00:00 [accepted]
PHST- 2008/05/14 09:00 [pubmed]
PHST- 2008/06/21 09:00 [medline]
PHST- 2008/05/14 09:00 [entrez]
AID - S0006-291X(08)00859-0 [pii]
AID - 10.1016/j.bbrc.2008.04.170 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Jul 18;372(1):73-7. doi: 
      10.1016/j.bbrc.2008.04.170. Epub 2008 May 12.