PMID- 18477815
OWN - NLM
STAT- MEDLINE
DCOM- 20080828
LR  - 20211020
IS  - 1741-2560 (Print)
IS  - 1741-2552 (Electronic)
IS  - 1741-2552 (Linking)
VI  - 5
IP  - 2
DP  - 2008 Jun
TI  - Modulation of cultured neural networks using neurotrophin release from 
      hydrogel-coated microelectrode arrays.
PG  - 203-13
LID - 10.1088/1741-2560/5/2/011 [doi]
AB  - Polyacrylamide and poly(ethylene glycol) diacrylate hydrogels were synthesized 
      and characterized for use as drug release and substrates for neuron cell culture. 
      Protein release kinetics was determined by incorporating bovine serum albumin 
      (BSA) into hydrogels during polymerization. To determine if hydrogel 
      incorporation and release affect bioactivity, alkaline phosphatase was 
      incorporated into hydrogels and a released enzyme activity determined using the 
      fluorescence-based ELF-97 assay. Hydrogels were then used to deliver a 
      brain-derived neurotrophic factor (BDNF) from hydrogels polymerized over planar 
      microelectrode arrays (MEAs). Primary hippocampal neurons were cultured on both 
      control and neurotrophin-containing hydrogel-coated MEAs. The effect of released 
      BDNF on neurite length and process arborization was investigated using automated 
      image analysis. An increased spontaneous activity as a response to the released 
      BDNF was recorded from the neurons cultured on the top of hydrogel layers. These 
      results demonstrate that proteins of biological interest can be incorporated into 
      hydrogels to modulate development and function of cultured neural networks. These 
      results also set the stage for development of hydrogel-coated neural prosthetic 
      devices for local delivery of various biologically active molecules.
FAU - Jun, Sang Beom
AU  - Jun SB
AD  - Nano-Bioelectronics and Systems Research Center, Seoul, Korea.
FAU - Hynd, Matthew R
AU  - Hynd MR
FAU - Dowell-Mesfin, Natalie M
AU  - Dowell-Mesfin NM
FAU - Al-Kofahi, Yousef
AU  - Al-Kofahi Y
FAU - Roysam, Badrinath
AU  - Roysam B
FAU - Shain, William
AU  - Shain W
FAU - Kim, Sung June
AU  - Kim SJ
LA  - eng
GR  - R01-NS044287/NS/NINDS NIH HHS/United States
GR  - R01 NS044287-04/NS/NINDS NIH HHS/United States
GR  - P41 EB002030-129003/EB/NIBIB NIH HHS/United States
GR  - R21 EB007782/EB/NIBIB NIH HHS/United States
GR  - R21 EB007782-02/EB/NIBIB NIH HHS/United States
GR  - R21EB007782/EB/NIBIB NIH HHS/United States
GR  - R01 NS044287/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080513
PL  - England
TA  - J Neural Eng
JT  - Journal of neural engineering
JID - 101217933
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Drug Carriers)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Action Potentials/*drug effects/*physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage/chemistry
MH  - Cell Culture Techniques/methods
MH  - Cells, Cultured
MH  - Coated Materials, Biocompatible/*administration & dosage/chemistry
MH  - Drug Carriers/chemistry
MH  - Hydrogels/chemistry
MH  - *Microelectrodes
MH  - Nerve Net/*drug effects/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC2767261
MID - NIHMS145966
EDAT- 2008/05/15 09:00
MHDA- 2008/08/30 09:00
PMCR- 2009/10/26
CRDT- 2008/05/15 09:00
PHST- 2008/05/15 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/05/15 09:00 [entrez]
PHST- 2009/10/26 00:00 [pmc-release]
AID - S1741-2560(08)72913-6 [pii]
AID - 10.1088/1741-2560/5/2/011 [doi]
PST - ppublish
SO  - J Neural Eng. 2008 Jun;5(2):203-13. doi: 10.1088/1741-2560/5/2/011. Epub 2008 May 
      13.