PMID- 18478926
OWN - NLM
STAT- MEDLINE
DCOM- 20100427
LR  - 20121115
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 29
IP  - 12
DP  - 2007 Dec
TI  - [Inhibiting effect of HPV16 E6 small interfering RNA on the growth of human 
      cervical carcinoma xenotransplanted in nude mice].
PG  - 894-7
AB  - OBJECTIVE: To explore the inhibiting effect of human papillomavirus 16 
      E6-specific small interfering RNA (siRNA) on cervical cancer transplanted in nude 
      mice. METHODS: CaSki cells transfected with HPV16 E6 siRNA were transplanted into 
      nude mice. HPV16 E6 siRNA was injected into the tumor, and the control group was 
      treated with NS. Tumor growth was monitored once every other day. Terminal 
      deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) was performed 
      to detect apoptosis. The expression of E6 and p53 protein was assessed by 
      immunohistochemistry. The contents of alanine aminotransferase (ALT) and 
      aspartate aminotransferase (AST) were measured and the liver and kidney were 
      examined by histopathology. RESULTS: Inhibition of tumor growth was demounstrated 
      after treatment with HPV16 E6 siRNA. The volume and weight of tumors were 
      significantly decreased in comparison with those of control group (P < 0.05). 
      Apoptosis occurred more frequently in the experiment group than in the control. 
      The expression of E6 and p53 was down-regulated. The contents of ALT and AST 
      underwent no significant changes, and the histopathology of liver and kidney 
      showed no abnormal changes. CONCLUSION: The growth ability of human cervical 
      cancer xenograft tumors in nude mice can be inhibited by HPV16 E6-specific siRNA, 
      with no toxic side effect on the liver. It may provide an useful method of 
      gene-therapy against cervical cancer.
FAU - Guan, Li-Li
AU  - Guan LL
AD  - Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan 
      University, Chengdu 610041, China.
FAU - Peng, Zhi-Lan
AU  - Peng ZL
FAU - Niu, Xiao-Yu
AU  - Niu XY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Repressor Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - *Apoptosis
MH  - Aspartate Aminotransferases/blood
MH  - Carcinoma, Squamous Cell/pathology
MH  - Cell Line, Tumor
MH  - Down-Regulation
MH  - Female
MH  - Genetic Therapy
MH  - Humans
MH  - Kidney/pathology
MH  - Liver/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Oncogene Proteins, Viral/*genetics/metabolism
MH  - *RNA Interference
MH  - RNA, Small Interfering/*genetics
MH  - Repressor Proteins/*genetics/metabolism
MH  - Transfection
MH  - Tumor Burden
MH  - Tumor Suppressor Protein p53/metabolism
MH  - Uterine Cervical Neoplasms/*pathology
MH  - Xenograft Model Antitumor Assays
EDAT- 2008/05/16 09:00
MHDA- 2010/04/28 06:00
CRDT- 2008/05/16 09:00
PHST- 2008/05/16 09:00 [pubmed]
PHST- 2010/04/28 06:00 [medline]
PHST- 2008/05/16 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2007 Dec;29(12):894-7.