PMID- 18480434
OWN - NLM
STAT- MEDLINE
DCOM- 20080801
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 82
IP  - 15
DP  - 2008 Aug
TI  - A bovine herpesvirus type 1 mutant virus specifying a carboxyl-terminal 
      truncation of glycoprotein E is defective in anterograde neuronal transport in 
      rabbits and calves.
PG  - 7432-42
LID - 10.1128/JVI.00379-08 [doi]
AB  - Bovine herpesvirus type 1 (BHV-1) is an important component of the bovine 
      respiratory disease complex (BRDC) in cattle. The ability of BHV-1 to transport 
      anterogradely from neuronal cell bodies in trigeminal ganglia (TG) to nerve 
      ending in the noses and corneas of infected cattle following reactivation from 
      latency plays a significant role in the pathogenesis of BRDC and maintenance of 
      BHV-1 in the cattle population. We have constructed a BHV-1 bacterial artificial 
      chromosome (BAC) clone by inserting an excisable BAC plasmid sequence in the long 
      intergenic region between the glycoprotein B (gB) and UL26 genes. A BAC-excised, 
      reconstituted BHV-1 containing only the 34-bp loxP sequence within the gB-UL26 
      intergenic region was highly infectious in calves, retained wild-type virulence 
      properties, and reactivated from latency following treatment with dexamethasone. 
      Using a two-step Red-mediated mutagenesis system in Escherichia coli, we 
      constructed a gE cytoplasmic tail-truncated BHV-1 and a gE-rescued BHV-1. 
      Following primary infection, the gE cytoplasmic tail-truncated virus was 
      efficiently transported retrogradely from the nerve endings in the nose and eye 
      to cell bodies in the TG of calves and rabbits. However, following 
      dexamethasone-induced reactivation from latency, the gE mutant virus was not 
      isolated from nasal and ocular sheddings. Reverse transcriptase PCR assays 
      detected VP5 transcription in the TG of rabbits infected with gE-rescued and gE 
      cytoplasmic tail-truncated viruses during primary infection and after 
      dexamethasone treatment but not during latency. Therefore, the BHV-1gE 
      cytoplasmic tail-truncated virus reactivated in the TG; however, it had defective 
      anterograde transport from TG to nose and eye in calves and rabbits.
FAU - Liu, Z F
AU  - Liu ZF
AD  - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, 
      Kansas State University, Manhattan, KS 66506, USA.
FAU - Brum, M C S
AU  - Brum MC
FAU - Doster, A
AU  - Doster A
FAU - Jones, C
AU  - Jones C
FAU - Chowdhury, S I
AU  - Chowdhury SI
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080514
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Mutant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (bovine herpesvirus type-1 glycoproteins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chromosomes, Artificial, Bacterial
MH  - Dexamethasone/administration & dosage
MH  - Eye/virology
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 1, Bovine/genetics/*pathogenicity
MH  - Infectious Bovine Rhinotracheitis/virology
MH  - Mutant Proteins/metabolism
MH  - Neurons/*virology
MH  - Nose/virology
MH  - Rabbits
MH  - Recombination, Genetic
MH  - Sequence Deletion
MH  - Trigeminal Ganglion/virology
MH  - Viral Envelope Proteins/genetics/*physiology
MH  - Viral Proteins
MH  - Virulence
MH  - Virus Activation
MH  - Virus Shedding
PMC - PMC2493312
EDAT- 2008/05/16 09:00
MHDA- 2008/08/02 09:00
PMCR- 2008/12/01
CRDT- 2008/05/16 09:00
PHST- 2008/05/16 09:00 [pubmed]
PHST- 2008/08/02 09:00 [medline]
PHST- 2008/05/16 09:00 [entrez]
PHST- 2008/12/01 00:00 [pmc-release]
AID - JVI.00379-08 [pii]
AID - 0379-08 [pii]
AID - 10.1128/JVI.00379-08 [doi]
PST - ppublish
SO  - J Virol. 2008 Aug;82(15):7432-42. doi: 10.1128/JVI.00379-08. Epub 2008 May 14.