PMID- 1848084
OWN - NLM
STAT- MEDLINE
DCOM- 19910416
LR  - 20121115
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 4
IP  - 3
DP  - 1991 Mar
TI  - Localization and induced expression of fusion genes in the rat lung.
PG  - 206-9
AB  - Liposome-mediated gene transfer is useful for DNA transfection into cells in 
      culture. We wondered whether this method could be used to introduce new DNA into 
      the intact lung. Fusion genes containing either the Rous sarcoma virus (RSV) 
      promoter or the mouse mammary tumor virus (MMTV) promoter (which contains 
      glucocorticoid response elements) were linked to the bacterial gene 
      chloramphenicol acetyltransferase (CAT), an enzyme not present in mammalian 
      cells. Plasmids containing the RSV-CAT fusion gene were mixed with cationic 
      liposomes (Lipofectin; BRL, Inc., Grand Island, NY), and single doses were 
      instilled into the cervical trachea of anesthetized rats. Control rats received 
      either liposomes or plasmid. After 24, 48, and 72 h, lungs were perfused free of 
      blood, homogenized, and analyzed for CAT enzyme activity. Liver and kidney tissue 
      were also obtained. We found that rats given either intratracheal liposomes or 
      plasmid had no detectable CAT activity. By contrast, 24 h after instillation of 
      lipid:DNA complexes, lung CAT expression remained elevated for the next 48 h but 
      was barely detectable in liver or kidney. In another group of rats, 
      MMTV-CAT:liposome complexes were instilled intratracheally and then the rats were 
      injected with either dexamethasone or saline. We found that the 
      dexamethasone-treated rats had a 5- to 10-fold higher level of lung CAT 
      expression at 24 and 48 h than the saline-treated controls had; liver and kidney 
      CAT levels were negligible in both groups. Dexamethasone treatment did not 
      increase RSV-CAT expression, indicating that the dexamethasone effect on MMTV-CAT 
      expression was related to the presence of the MMTV promoter.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Hazinski, T A
AU  - Hazinski TA
AD  - Vanderbilt University School of Medicine, Department of Pediatrics, Nashville, 
      Tennessee.
FAU - Ladd, P A
AU  - Ladd PA
FAU - DeMatteo, C A
AU  - DeMatteo CA
LA  - eng
GR  - HL-14214/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (DNA, Recombinant)
RN  - 0 (Phosphatidylethanolamines)
RN  - 76391-83-8 (1,2-dielaidoylphosphatidylethanolamine)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Avian Sarcoma Viruses/genetics
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis/genetics
MH  - Cytomegalovirus/genetics
MH  - DNA, Recombinant/administration & dosage
MH  - Epithelium/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Genetic Therapy/*methods
MH  - Genetic Vectors
MH  - Lung/*metabolism
MH  - Male
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Phosphatidylethanolamines
MH  - Rats
MH  - beta-Galactosidase/biosynthesis/genetics
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - 10.1165/ajrcmb/4.3.206 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1991 Mar;4(3):206-9. doi: 10.1165/ajrcmb/4.3.206.