PMID- 18480845
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20201222
IS  - 1476-5462 (Electronic)
IS  - 0969-7128 (Linking)
VI  - 15
IP  - 19
DP  - 2008 Oct
TI  - Engineering of highly immunogenic long-lived DC vaccines by antiapoptotic protein 
      gene transfer to enhance cancer vaccine potency.
PG  - 1321-9
LID - 10.1038/gt.2008.85 [doi]
AB  - Dendritic cells (DCs) have a critical role in the induction of antigen-specific 
      immune responses, transporting antigens from peripheral tissue to regional lymph 
      nodes where they interact with antigen-specific T lymphocytes. Recent studies 
      revealed that the efficacy of the T cell-dependent immune response depends on the 
      lifespan of the antigen-presenting DCs in the lymph nodes. Here, we succeeded in 
      engineering long-lived antigen-presenting DCs via Bcl-XL-derived hyperactive 
      mutant antiapoptotic protein (Bcl-X FNK) gene transfer. In a B16BL6 melanoma 
      model, these long-lived DCs exerted potent antitumor immunity that depended 
      mainly on antigen-specific cytotoxic T lymphocytes. Furthermore, in vivo 
      longevity of the long-lived DC vaccine led to antigen-specific activation of 
      interferon-gamma-producing CD4+ and CD8+ T cells. Thus, the long-lived DC vaccine 
      strategy is highly useful for constructing DC vaccines, as well as other 
      cell-based medicines, such as stem cell therapy.
FAU - Yoshikawa, T
AU  - Yoshikawa T
AD  - Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical 
      Sciences, Osaka University, Suita, Osaka, Japan.
FAU - Niwa, T
AU  - Niwa T
FAU - Mizuguchi, H
AU  - Mizuguchi H
FAU - Okada, N
AU  - Okada N
FAU - Nakagawa, S
AU  - Nakagawa S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080515
PL  - England
TA  - Gene Ther
JT  - Gene therapy
JID - 9421525
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Movement
MH  - Cell Survival
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Genetic Engineering
MH  - Genetic Therapy/*methods
MH  - Immunotherapy, Adoptive/*methods
MH  - Interferon-gamma/immunology
MH  - Melanoma, Experimental/immunology/*therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - Skin Neoplasms/immunology/*therapy
MH  - Transduction, Genetic/methods
EDAT- 2008/05/16 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/05/16 09:00
PHST- 2008/05/16 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/05/16 09:00 [entrez]
AID - gt200885 [pii]
AID - 10.1038/gt.2008.85 [doi]
PST - ppublish
SO  - Gene Ther. 2008 Oct;15(19):1321-9. doi: 10.1038/gt.2008.85. Epub 2008 May 15.