PMID- 18481228
OWN - NLM
STAT- MEDLINE
DCOM- 20080820
LR  - 20211020
IS  - 1355-008X (Print)
IS  - 1355-008X (Linking)
VI  - 33
IP  - 2
DP  - 2008 Apr
TI  - The impact of unabated stimulation by human chorionic gonadotropin on the steroid 
      hormone environment of pregnant rats and the spontaneous expression of ovarian 
      cysts in female progeny.
PG  - 152-64
LID - 10.1007/s12020-008-9072-z [doi]
AB  - Unabated stimulation by low doses of human chorionic gonadotropin (hCG) induces 
      ovarian cysts in pregnant rats. In order to determine the impact of these in vivo 
      treatments on the hormonal milieu of pregnancy, and the potential impact of an 
      aberrant cystic-ovary state during pregnancy on the resulting female offspring, 
      pregnant rats were treated with either 0 (control), 1, or 3 IU hCG twice daily 
      for at least 9 days, beginning on day 13 of pregnancy. Serum was harvested from 
      control and hCG treated animals on days 15, 17, 19, and 22 of pregnancy. Control 
      pregnant rats and animals treated with 1 IU hCG shared similar serum profiles for 
      progesterone (P4), androstenedione (A4), 5alpha-androstane-3alpha,17beta-diol 
      (3alpha-diol), androsterone (A5), and estrone (E1) between days 15 and 22 of 
      pregnancy. Testosterone serum concentrations were similar for control and 1 IU 
      hGG-treated pregnant rats between days 15 and 19 of pregnancy; whereas, on day 
      22, 1 IU hGG-treated pregnant rats displayed lower serum testosterone than 
      control pregnant rats (P < or = 0.05). In contrast, serum estradiol (E2) 
      concentrations for 1 IU hCG- treated pregnant rats were greater than E2 values 
      observed for control rats on days 15-19 of pregnancy (P < or = 0.05). Serum 
      testosterone and 3alpha-diol values for 3 IU hCG-treated pregnant rats differed 
      from those of control pregnant rats only on day 19 when these values were 
      transiently greater for these hCG-treated animals compared with serum values for 
      control pregnant rats (P < or = 0.05). Serum A4 values for 3 IU hCG-treated 
      pregnant rats were elevated compared to values for control pregnant rats only on 
      days 15 and 17 (P < or = 0.05). In contrast, serum E1, A5, and E2 were elevated 
      on days 19-22, 17-22, and 15-22, respectively, in 3 IU hCG-treated pregnant rats 
      compared to control pregnant rats (P < or = 0.05). No pups from control pregnant 
      rats displayed ovarian cysts during the time they were observed postnatally. In 
      contrast, 6 of 25 pups from 3 IU hCG-treated pregnant rats displayed cystic 
      ovaries, without corpora lutea, on day 55 of age. Serum steroid concentrations 
      for these cyst-bearing progeny were similar to those of female progeny from 
      control pregnant rats, whereas female progeny without ovarian cysts from 3 IU 
      hCG-treated pregnant rats displayed differences in serum steroid values from 
      those of progeny from control pregnant rats (P < or = 0.05). The data support the 
      concept that an aberrant, yet physiologic hormonal environment associated with 
      the induction of ovarian cysts during pregnancy in rats, can lead to the 
      spontaneous establishment of an ovarian cystic state in at least a subset of the 
      female progeny. Further, the date suggest that tonically increased ovarian 
      estrogen production during pregnancy, reflected by tonically elevated peripheral 
      serum estrogen concentrations, may play a pivotal role in the etiology of an 
      ovarian cystic state in this subset of daughters from hCG-induced, cyst-bearing 
      pregnant rats.
FAU - Bogovich, Katryna
AU  - Bogovich K
AD  - The University of South Carolina, School of Medicine, Building 28, First Floor, 
      Columbia, SC 29208, USA. bogovich@gw.med.sc.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080515
PL  - United States
TA  - Endocrine
JT  - Endocrine
JID - 9434444
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Insulin)
RN  - 0 (Steroids)
RN  - 2DI9HA706A (Estrone)
RN  - 3XMK78S47O (Testosterone)
RN  - 409J2J96VR (Androstenedione)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - C24W7J5D5R (Androsterone)
SB  - IM
MH  - Aging/metabolism
MH  - Androstenedione/blood
MH  - Androsterone/blood
MH  - Animals
MH  - Chorionic Gonadotropin/*pharmacology
MH  - Estradiol/blood
MH  - Estrone/blood
MH  - Female
MH  - Insulin/blood
MH  - Ovarian Cysts/*chemically induced/pathology
MH  - Pregnancy
MH  - Pregnancy, Animal/*metabolism
MH  - Progesterone/blood
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Steroids/*metabolism
MH  - Stimulation, Chemical
MH  - Testosterone/blood
MH  - Uterus/drug effects/metabolism
EDAT- 2008/05/16 09:00
MHDA- 2008/08/21 09:00
CRDT- 2008/05/16 09:00
PHST- 2008/02/19 00:00 [received]
PHST- 2008/04/22 00:00 [accepted]
PHST- 2008/03/28 00:00 [revised]
PHST- 2008/05/16 09:00 [pubmed]
PHST- 2008/08/21 09:00 [medline]
PHST- 2008/05/16 09:00 [entrez]
AID - 10.1007/s12020-008-9072-z [doi]
PST - ppublish
SO  - Endocrine. 2008 Apr;33(2):152-64. doi: 10.1007/s12020-008-9072-z. Epub 2008 May 
      15.