PMID- 18482994
OWN - NLM
STAT- MEDLINE
DCOM- 20080929
LR  - 20161124
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 23
IP  - 8
DP  - 2008 Aug
TI  - The relationship between meiotic recombination in human spermatocytes and 
      aneuploidy in sperm.
PG  - 1691-7
LID - 10.1093/humrep/den027 [doi]
AB  - BACKGROUND: We have previously demonstrated that a decreased recombination 
      frequency between human X and Y chromosomes is associated with the production of 
      aneuploid 24,XY sperm. This study's aim was to determine the relationship between 
      recombination frequency in human pachytene spermatocytes and aneuploidy 
      frequencies in individual chromosomes in sperm from the same men. METHODS: Six 
      previously fertile vasectomy reversal patients donated testicular tissue for 
      meiotic analysis of pachytene spermatocytes using immunocytogenetic techniques 
      for visualization of the synaptonemal complex and recombination sites (MLH1). 
      Individual meiotic chromosomes were identified with centromere-specific 
      multicolor fluorescence in situ hybridization (FISH), and the number of MLH1 
      signals was recorded for individual chromosomes. An ejaculated sperm sample was 
      obtained from each patient 2-26 months post-reversal for FISH analysis of sperm 
      aneuploidy frequencies of chromosomes 1, 9, 13, 21, X and Y. RESULTS: There was 
      no significant correlation between meiotic recombination frequency and sperm 
      aneuploidy for any individual chromosome. Similarly, there was no correlation 
      between aneuploid sperm and bivalents with no recombination. CONCLUSIONS: The 
      study provides unique data on intra-individual human recombination and aneuploidy 
      events. It also demonstrated for the first time that men do not have an increased 
      frequency of sperm aneuploidy 5-9 years post-vasectomy.
FAU - Sun, F
AU  - Sun F
AD  - Department of Medical Genetics, University of Calgary, 3330 Hospital Dr, NW, 
      Calgary, AB, Canada.
FAU - Mikhaail-Philips, M
AU  - Mikhaail-Philips M
FAU - Oliver-Bonet, M
AU  - Oliver-Bonet M
FAU - Ko, E
AU  - Ko E
FAU - Rademaker, A
AU  - Rademaker A
FAU - Turek, P
AU  - Turek P
FAU - Martin, R H
AU  - Martin RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080515
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (MLH1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - EC 3.6.1.3 (MutL Protein Homolog 1)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/analysis
MH  - Adult
MH  - *Aneuploidy
MH  - Humans
MH  - Male
MH  - Meiosis/*genetics
MH  - Middle Aged
MH  - MutL Protein Homolog 1
MH  - Nuclear Proteins/analysis
MH  - *Recombination, Genetic
MH  - Spermatocytes/*cytology/ultrastructure
MH  - Vasovasostomy
EDAT- 2008/05/17 09:00
MHDA- 2008/09/30 09:00
CRDT- 2008/05/17 09:00
PHST- 2008/05/17 09:00 [pubmed]
PHST- 2008/09/30 09:00 [medline]
PHST- 2008/05/17 09:00 [entrez]
AID - den027 [pii]
AID - 10.1093/humrep/den027 [doi]
PST - ppublish
SO  - Hum Reprod. 2008 Aug;23(8):1691-7. doi: 10.1093/humrep/den027. Epub 2008 May 15.