PMID- 18484240
OWN - NLM
STAT- MEDLINE
DCOM- 20090818
LR  - 20211020
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 88
IP  - 1
DP  - 2008 Jul
TI  - Phagocytosis of co-developing neutrophil progenitors by dendritic cells in a 
      culture of human CD34(+) cells with granulocyte colony-stimulating factor and 
      tumor necrosis factor-alpha.
PG  - 64-72
LID - 10.1007/s12185-008-0098-z [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been shown to induce the 
      differentiation of CD34(+) cells toward dendritic cells (DCs). We have previously 
      shown that DCs are co-generated from human CD34(+) cells during erythroid or 
      megakaryocytic differentiation in the presence of TNF-alpha, and those DCs are 
      able to stimulate autologous T cell proliferation. The aim of this study was to 
      learn whether the co-stimulation of granulocyte colony-stimulating factor (G-CSF) 
      and TNF-alpha would generate neutrophil progenitors and DCs together from human 
      CD34(+) cells, and if this was the case, to clarify the phenotypic and functional 
      characteristics of these DCs. When highly purified human CD34(+) cells were 
      cultured for 7 days with G-CSF alone, the generated cells predominantly expressed 
      a granulocyte marker, CD15, and then differentiated into neutrophils after 14 
      days of culture. The addition of TNF-alpha with G-CSF markedly decreased the 
      number of CD15(+) cells without affecting the total number of cells during 7 days 
      of culture. Almost one third of the generated cells were positive for CD11c and 
      CD123. Furthermore, CD11c(+) cells were found to phagocytose CD15(+) cells and 
      were able to induce allogeneic, but not autologous, T cell proliferation in the 
      mixed lymphocyte reaction (MLR). On the other hand, the CD11c(+) cells generated 
      by TNF-alpha and cytokines capable of inducing erythroid differentiation were 
      able to stimulate autologous T cells. There was a difference in the expression of 
      CD80, CD83 and CD86 among CD11c(+) cells induced by G-CSF plus TNF-alpha and 
      those generated by interleukin-3, stem cell factor, and erythropoietin plus 
      TNF-alpha. These results indicate that the co-stimulation of human CD34(+) cells 
      with G-CSF and TNF-alpha induces the phagocytosis of co-developing neutrophil 
      progenitors by DCs, and the stimulatory effects of these DCs on autologous T 
      cells is different from that of DCs generated from CD34(+) cells during erythroid 
      differentiation.
FAU - Saito, Yoshinobu
AU  - Saito Y
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Guo, Yong Mei
AU  - Guo YM
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Hirokawa, Makoto
AU  - Hirokawa M
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Saito, Kunie
AU  - Saito K
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Komatsuda, Atsushi
AU  - Komatsuda A
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Takahashi, Naoto
AU  - Takahashi N
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Fujishima, Masumi
AU  - Fujishima M
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Fujishima, Naohito
AU  - Fujishima N
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan.
FAU - Yamashita, Junsuke
AU  - Yamashita J
AD  - Radioisotope Division, Bioscience center, Akita University Graduate School of 
      Medicine, Akita, 010-8543, Japan.
FAU - Sawada, Kenichi
AU  - Sawada K
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita, 010-8543, Japan. ksawada@doc.med.akita-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080520
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - *Antigens, CD34
MH  - Antigens, Differentiation/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*metabolism
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - Granulocyte Precursor Cells/cytology/*metabolism
MH  - Humans
MH  - Megakaryocyte Progenitor Cells/cytology/*metabolism
MH  - Megakaryocytes/cytology/*metabolism
MH  - Neutrophils/cytology/*metabolism
MH  - Phagocytosis/*drug effects/physiology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2008/05/20 09:00
MHDA- 2009/08/19 09:00
CRDT- 2008/05/20 09:00
PHST- 2008/01/29 00:00 [received]
PHST- 2008/04/04 00:00 [accepted]
PHST- 2008/03/09 00:00 [revised]
PHST- 2008/05/20 09:00 [pubmed]
PHST- 2009/08/19 09:00 [medline]
PHST- 2008/05/20 09:00 [entrez]
AID - 10.1007/s12185-008-0098-z [pii]
AID - 10.1007/s12185-008-0098-z [doi]
PST - ppublish
SO  - Int J Hematol. 2008 Jul;88(1):64-72. doi: 10.1007/s12185-008-0098-z. Epub 2008 
      May 20.