PMID- 18485095
OWN - NLM
STAT- MEDLINE
DCOM- 20080915
LR  - 20080807
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 106
IP  - 3
DP  - 2008 Aug
TI  - Regulatory role of neuron-restrictive silencing factor in the specific expression 
      of cocaine- and amphetamine-regulated transcript gene.
PG  - 1314-24
LID - 10.1111/j.1471-4159.2008.05487.x [doi]
AB  - Cocaine- and amphetamine-regulated transcript (CART) peptide is an endogenous 
      peptide which is widely expressed in the CNS and PNS as well as in endocrine 
      cells. Despite the functional knowledge about CART, the mechanisms that regulate 
      CART gene transcription are poorly characterized. Here, we showed that 
      neuron-restrictive silencer factor (NRSF) functions as a negative regulator of 
      CART gene expression in neuroendocrine cells. A putative neuron-restrictive 
      silencer element (NRSE) conserved between the rodent and human CART promoter was 
      identified and demonstrated to bind to NRSF in sequence-specific manner by the 
      electrophoretic mobility shift and chromatin immunoprecipitation assays. Ectopic 
      expression of NRSF in pheochromocytoma cells (PC12) and insulin-secreting cells 
      (INS-1) induced a marked reduction in the level of CART mRNA and the activity of 
      CART promoter or NRSE reporter. The CART promoter showed very low activity in 
      endogenous NRSF-expressing HeLa cells. When expression of NRSF was down-regulated 
      in HeLa cells using a RNA interfering technique, the transcriptional activity of 
      the CART promoter or a NRSE reporter was significantly increased. Taken together, 
      our data suggested that CART gene expression in neuroendocrine cells is strictly 
      controlled by NRSF, via a mechanism dependent upon the CART NRSE.
FAU - Li, Yanhua
AU  - Li Y
AD  - Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion 
      Medicine, Beijing, China.
FAU - Liu, Qingbin
AU  - Liu Q
FAU - Yang, Yinxiang
AU  - Yang Y
FAU - Lv, Yang
AU  - Lv Y
FAU - Chen, Lin
AU  - Chen L
FAU - Bai, Cixian
AU  - Bai C
FAU - Nan, Xue
AU  - Nan X
FAU - Wang, Yunfang
AU  - Wang Y
FAU - Pei, Xuetao
AU  - Pei X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080515
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (Repressor Proteins)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Gene Expression Regulation/*physiology
MH  - Gene Silencing/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - Neurosecretory Systems/cytology/metabolism/*physiology
MH  - PC12 Cells
MH  - Protein Binding/physiology
MH  - Rats
MH  - Repressor Proteins/*physiology
EDAT- 2008/05/20 09:00
MHDA- 2008/09/16 09:00
CRDT- 2008/05/20 09:00
PHST- 2008/05/20 09:00 [pubmed]
PHST- 2008/09/16 09:00 [medline]
PHST- 2008/05/20 09:00 [entrez]
AID - JNC5487 [pii]
AID - 10.1111/j.1471-4159.2008.05487.x [doi]
PST - ppublish
SO  - J Neurochem. 2008 Aug;106(3):1314-24. doi: 10.1111/j.1471-4159.2008.05487.x. Epub 
      2008 May 15.