PMID- 18485691
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20191210
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 24
IP  - 2
DP  - 2008 Oct 15
TI  - Direct electrochemistry of horseradish peroxidase on TiO(2) nanotube arrays via 
      seeded-growth synthesis.
PG  - 198-203
LID - 10.1016/j.bios.2008.03.031 [doi]
AB  - Horseradish peroxidase (HRP) was successfully immobilized on vertically oriented 
      TiO(2) nanotube arrays (NTAs), which was prepared by a seeded-growth mechanism. 
      The nanotubular structure of TiO(2) was characterized by scanning electron 
      microscope (SEM). After encapsulated HRP on TiO(2) nanotube arrays, the direct 
      electron transfer of HRP was observed. Owing to the redox reaction of 
      electroactive center of HRP, the HRP/TiO(2) NTAs modified electrode exhibited a 
      pair of quasi-reversible peaks with the peak-to-peak separation of 70 mV and the 
      formal potential of -0.122 V (vs. SCE) in 0.2mol L(-1) phosphate buffer solution 
      (PBS, pH 7.0). The number of transference electron was 0.84 and the direct 
      electron transfer (ET) constant (k(s)) was 3.82 s(-1). The HRP/TiO(2) NTAs 
      modified electrode displayed an excellent electrocatalytic performance for 
      H(2)O(2) and the formal Michaelis-Menten constant (K(m)(app)) was 1.9 mmol L(-1). 
      The response currents had a good linear relation with the concentration of 
      H(2)O(2) from 5.0 x 10(-7) mol L(-1) to 1.0 x 10(-5) mol L(-1) and 5.0 x 10(-5) 
      mol L(-1) to 1.0 x10(-3) mol L(-1), respectively.
FAU - Wu, Fanghua
AU  - Wu F
AD  - Department of Chemistry, East China Normal University, Shanghai 200062, China.
FAU - Xu, Jingjing
AU  - Xu J
FAU - Tian, Yuan
AU  - Tian Y
FAU - Hu, Zhichao
AU  - Hu Z
FAU - Wang, Liwei
AU  - Wang L
FAU - Xian, Yuezhong
AU  - Xian Y
FAU - Jin, Litong
AU  - Jin L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080408
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Coated Materials, Biocompatible)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - D1JT611TNE (Titanium)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - *Biosensing Techniques
MH  - Coated Materials, Biocompatible/chemistry
MH  - Crystallization/methods
MH  - Electrochemistry/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Horseradish Peroxidase/*chemistry
MH  - Hydrogen Peroxide/*analysis
MH  - Nanotubes/*chemistry/ultrastructure
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Titanium/*chemistry
EDAT- 2008/05/20 09:00
MHDA- 2008/11/19 09:00
CRDT- 2008/05/20 09:00
PHST- 2007/11/28 00:00 [received]
PHST- 2008/03/02 00:00 [revised]
PHST- 2008/03/25 00:00 [accepted]
PHST- 2008/05/20 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/05/20 09:00 [entrez]
AID - S0956-5663(08)00157-7 [pii]
AID - 10.1016/j.bios.2008.03.031 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2008 Oct 15;24(2):198-203. doi: 10.1016/j.bios.2008.03.031. 
      Epub 2008 Apr 8.