PMID- 18485891
OWN - NLM
STAT- MEDLINE
DCOM- 20080620
LR  - 20161124
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 372
IP  - 1
DP  - 2008 Jul 18
TI  - HGF/SF and menthol increase human glioblastoma cell calcium and migration.
PG  - 210-5
LID - 10.1016/j.bbrc.2008.05.032 [doi]
AB  - This study explored the role of transient receptor potential melastatin 8 ion 
      channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. 
      Menthol stimulated influx of Ca(2+), membrane current, and migration of DBTRG 
      cells. Effects on Ca(2+) and migration were enhanced by pre-treatment with 
      hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca(2+) also were 
      greater in migrating cells compared with non-migrating cells. 
      2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR 
      and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein 
      for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma 
      membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma 
      membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase 
      [Ca(2+)](i) needed for DBTRG cell migration.
FAU - Wondergem, Robert
AU  - Wondergem R
AD  - Department of Physiology, James H. Quillen College of Medicine, East Tennessee 
      State University, P.O. Box 70,576, Johnson City, TN 37614-1708, USA. 
      wonderge@etsu.edu
FAU - Ecay, Tom W
AU  - Ecay TW
FAU - Mahieu, Frank
AU  - Mahieu F
FAU - Owsianik, Grzegorz
AU  - Owsianik G
FAU - Nilius, Bernd
AU  - Nilius B
LA  - eng
PT  - Journal Article
DEP - 20080515
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Boron Compounds)
RN  - 0 (HGF protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (TRPM Cation Channels)
RN  - 0 (TRPM8 protein, human)
RN  - 1490-04-6 (Menthol)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - E4ES684O93 (2-aminoethoxydiphenyl borate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Boron Compounds/pharmacology
MH  - Calcium/*metabolism
MH  - *Cell Movement/drug effects
MH  - Central Nervous System Neoplasms/metabolism/*pathology
MH  - Glioblastoma/metabolism/*pathology
MH  - Hepatocyte Growth Factor/*metabolism/pharmacology
MH  - Humans
MH  - Menthol/*toxicity
MH  - Neoplasm Invasiveness
MH  - RNA, Messenger/analysis/metabolism
MH  - TRPM Cation Channels/agonists/genetics/*metabolism
EDAT- 2008/05/20 09:00
MHDA- 2008/06/21 09:00
CRDT- 2008/05/20 09:00
PHST- 2008/04/30 00:00 [received]
PHST- 2008/05/05 00:00 [accepted]
PHST- 2008/05/20 09:00 [pubmed]
PHST- 2008/06/21 09:00 [medline]
PHST- 2008/05/20 09:00 [entrez]
AID - S0006-291X(08)00919-4 [pii]
AID - 10.1016/j.bbrc.2008.05.032 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Jul 18;372(1):210-5. doi: 
      10.1016/j.bbrc.2008.05.032. Epub 2008 May 15.