PMID- 18486181 OWN - NLM STAT- MEDLINE DCOM- 20081031 LR - 20101118 IS - 0043-1354 (Print) IS - 0043-1354 (Linking) VI - 42 IP - 13 DP - 2008 Jul TI - A universal threshold concept for hydrophobic mycolata in activated sludge foaming. PG - 3446-54 LID - 10.1016/j.watres.2008.02.033 [doi] AB - Recent studies using quantitative fluorescence in situ hybridization (FISH) have supported the principle that there are mycolata concentration thresholds, above which foaming is likely to occur. In this study, we surveyed 14 wastewater treatment plants (WWTPs) in the UK, using quantitative FISH, to establish that the principle of a mycolata threshold (2 x 10(6) mycolata cells ml(-1) mixed liquor suspended solids) is an empirical though widely held value. In addition, we designed, optimized and applied probes for members of the less hydrophobic mycolata genera Corynebacterium and Dietzia, to show that these organisms dominated the mycolata populations in two non-foaming WWTPs where the mycolata concentrations were above the threshold value. We propose that the mycolata threshold value is only applicable to hydrophobic members of the mycolata. FAU - Davenport, R J AU - Davenport RJ AD - School of Civil Engineering and Geosciences, Cassie Building, University of Newcastle, Newcastle upon Tyne, UK. r.j.davenport@ncl.ac.uk FAU - Pickering, R L AU - Pickering RL FAU - Goodhead, A K AU - Goodhead AK FAU - Curtis, T P AU - Curtis TP LA - eng PT - Journal Article DEP - 20080318 PL - England TA - Water Res JT - Water research JID - 0105072 RN - 0 (RNA, Ribosomal) RN - 0 (Sewage) SB - IM MH - Actinomycetales/*chemistry/genetics/*metabolism MH - *Hydrophobic and Hydrophilic Interactions MH - In Situ Hybridization, Fluorescence MH - RNA, Ribosomal/genetics MH - Sewage/*chemistry/*microbiology EDAT- 2008/05/20 09:00 MHDA- 2008/11/01 09:00 CRDT- 2008/05/20 09:00 PHST- 2007/08/14 00:00 [received] PHST- 2008/02/28 00:00 [revised] PHST- 2008/02/29 00:00 [accepted] PHST- 2008/05/20 09:00 [pubmed] PHST- 2008/11/01 09:00 [medline] PHST- 2008/05/20 09:00 [entrez] AID - S0043-1354(08)00111-5 [pii] AID - 10.1016/j.watres.2008.02.033 [doi] PST - ppublish SO - Water Res. 2008 Jul;42(13):3446-54. doi: 10.1016/j.watres.2008.02.033. Epub 2008 Mar 18.