PMID- 18488331
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20080519
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 14
IP  - 3
DP  - 2008 Mar
TI  - [An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia 
      patients by triple-color fluorescence in situ hybridization].
PG  - 211-4
AB  - OBJECTIVE: To analyze the numerical aberration of chromosome X, Y and 18 in the 
      spermatozoa of asthenospermia patients by triple-color fluorescence in situ 
      hybridization. METHODS: The experiment included 10 asthenospermia patients and 5 
      healthy men with normal semen quality as controls. Fluorescence in situ 
      hybridization (FISH) and probes for chromosomes including X, Y and 18 were used 
      to determine the frequency of the aneuploid of the chromosomes in spermatozoa. 
      RESULTS: Of the 45,547 spermatozoa counted from the semen samples, the 
      hybridization rate was 99.18%. The frequencies of the chromosome disomies 
      including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 
      0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the 
      case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 
      +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 
      9.25% in the former and 2.70% in the latter, with significant difference in 
      between (P< 0.01). CONCLUSION: Asthenospermia patients have a higher aneuploid 
      rate of sperm chromosome than normal fertile men. However, larger samples are yet 
      to be studied to obtain more scientific evidence.
FAU - Li, Lian-Bing
AU  - Li LB
AD  - Chongqing Institute of Science and Technology for National Population and Family 
      Planning, Chongqing 400020, China.
FAU - Xia, Yan-Kai
AU  - Xia YK
FAU - Li, Xin-Sheng
AU  - Li XS
FAU - Lu, Jing
AU  - Lu J
FAU - Ma, Ming-Fu
AU  - Ma MF
FAU - Song, Ling
AU  - Song L
FAU - Ji, Yuan
AU  - Ji Y
FAU - Yang, Ji-Gao
AU  - Yang JG
FAU - Zhang, Tian-Feng
AU  - Zhang TF
FAU - Chen, Xiao-Xing
AU  - Chen XX
FAU - Rong, Ying
AU  - Rong Y
FAU - Zhao, Le-Tian
AU  - Zhao LT
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
SB  - IM
MH  - Aneuploidy
MH  - Asthenozoospermia/*genetics
MH  - Chromosome Painting/*methods
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Humans
MH  - Male
MH  - Sex Chromosome Aberrations
MH  - Spermatozoa/metabolism
EDAT- 2008/05/21 09:00
MHDA- 2008/10/28 09:00
CRDT- 2008/05/21 09:00
PHST- 2008/05/21 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/05/21 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2008 Mar;14(3):211-4.