PMID- 1848868
OWN - NLM
STAT- MEDLINE
DCOM- 19910501
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 29
IP  - 2
DP  - 1991 Feb
TI  - Several groups among human herpesvirus 6 strains can be distinguished by Southern 
      blotting and polymerase chain reaction.
PG  - 367-72
AB  - Eight human herpesvirus 6 (HHV-6) strains were studied by Southern blot and 
      polymerase chain reaction. DNA from infected cells was digested by a panel of 
      restriction enzymes and hybridized with cloned BamHI fragments corresponding to 
      about 30% of the HHV-6 strain SIE genome. In parallel, this DNA was amplified by 
      polymerase chain reaction using pairs of primers derived from the strain SIE 
      nucleotide sequence. Subsequently, amplification products were analyzed by 
      hybridization, digestion with restriction endonucleases, and partial nucleotide 
      sequencing. Overall results indicated that all strains were closely related to 
      one another. However, concordant differences in restriction patterns allowed at 
      least two groups to be distinguished, typified by strains SIE and HST, 
      respectively. Differences between the two groups were found to reflect a limited 
      number of punctual changes in nucleotide sequences. These results strengthen the 
      idea of a unique HHV-6 species with genetic polymorphism. In addition, this study 
      provides useful markers for the diagnosis and molecular epidemiology of HHV-6 
      infections.
FAU - Aubin, J T
AU  - Aubin JT
AD  - Laboratoire de Bacteriologie-Virologie, C.E.R.V.I., Hopital de la 
      Pitie-Salpetriere, Paris, France.
FAU - Collandre, H
AU  - Collandre H
FAU - Candotti, D
AU  - Candotti D
FAU - Ingrand, D
AU  - Ingrand D
FAU - Rouzioux, C
AU  - Rouzioux C
FAU - Burgard, M
AU  - Burgard M
FAU - Richard, S
AU  - Richard S
FAU - Huraux, J M
AU  - Huraux JM
FAU - Agut, H
AU  - Agut H
LA  - eng
SI  - GENBANK/M58512
SI  - GENBANK/M58513
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
EIN - J Clin Microbiol 1992 Sep;30(9):2524
EIN - J Clin Microbiol. 1992 Sep;30(9):2524. PMID: 1328291
MH  - Base Sequence
MH  - Blotting, Southern
MH  - DNA, Viral/genetics
MH  - Herpesviridae Infections/diagnosis/microbiology
MH  - Herpesvirus 6, Human/classification/*genetics/isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Restriction Mapping
MH  - Species Specificity
PMC - PMC269769
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
PMCR- 1991/02/01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
PHST- 1991/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.29.2.367-372.1991 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1991 Feb;29(2):367-72. doi: 10.1128/jcm.29.2.367-372.1991.