PMID- 18490175
OWN - NLM
STAT- MEDLINE
DCOM- 20080728
LR  - 20190816
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 42
IP  - 3
DP  - 2008 Jun
TI  - House dust mite, Dermatophagoides pteronissinus increases expression of MCP-1, 
      IL-6, and IL-8 in human monocytic THP-1 cells.
PG  - 365-71
LID - 10.1016/j.cyto.2008.03.010 [doi]
AB  - The house dust mite (Dermatophagoides pteronissinus) plays an important role in 
      the pathogenesis of allergic diseases, including atopic dermatitis, and asthma. 
      Monocyte chemotactic protein 1 (MCP-1/CCL2)/IL-6/IL-8 (CXCL8) plays a pivotal 
      role in mediating the infiltration of various cells into the skin of atopic 
      dermatitis and psoriasis. The aim of this study was to investigate the effect of 
      D. pteronissinus extract (DpE) on expression of MCP-1/IL-6/IL-8 mRNA and protein 
      and the signal transduction in the human monocytic cell line, THP-1. The mRNA and 
      protein expression of MCP-1/CCL2, IL-6, and IL-8 were elevated by DpE in a time 
      and dose-dependent manner in THP-1 cells. The increased expression of MCP-1, 
      IL-6, and IL-8 was not affected by aprotinin (serine protease inhibitor) or E64 
      (cysteine protease inhibitor). We found that MCP-1 and IL-6 expression due to DpE 
      was related to Src, protein kinase C delta (PKC delta), 
      extracellular-signal-regulated kinase (ERK) and p38 mitogen-activated protein 
      kinase (MAPK) and IL-8 expression was involved in Src family tyrosine kinase, PKC 
      delta, ERK. DpE increased the phosphorylation of ERK and p38 MAPK after 5min and 
      peaked at 30min. The activation was significantly blocked by PP2, an inhibitor of 
      Src family tyrosine kinase and rottlerin, an inhibitor of PKC delta (p<0.01). DpE 
      increases MCP-1, IL-6, and IL-8 expression and transduces its signal via Src 
      family tyrosine kinase, PKC, and ERK in a protease-independent manner. This 
      finding may contribute to the elucidation of the pathogenic mechanism triggered 
      by DpE .
FAU - Lee, Ji-Sook
AU  - Lee JS
AD  - Department of Biology, Daejeon University, 96-3 Yongun-dong, Dong-gu, Daejeon 
      300-716, Republic of Korea.
FAU - Kim, In Sik
AU  - Kim IS
FAU - Ryu, Ji-Sun
AU  - Ryu JS
FAU - Yun, Chi-Young
AU  - Yun CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080519
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (AG 1879)
RN  - 0 (Acetophenones)
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Benzopyrans)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Pyrimidines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - E29LP3ZMUH (rottlerin)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C-delta)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Acetophenones/pharmacology
MH  - Allergens/*immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Benzopyrans/pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/biosynthesis
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Dermatophagoides pteronyssinus/*immunology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Humans
MH  - Interleukin-6/biosynthesis
MH  - Interleukin-8/biosynthesis
MH  - Monocytes/drug effects/*metabolism
MH  - Phosphorylation
MH  - Protein Kinase C-delta/metabolism
MH  - Pyrimidines/pharmacology
MH  - RNA, Messenger/analysis
MH  - Serine Proteinase Inhibitors/pharmacology
MH  - *Signal Transduction
MH  - Time Factors
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
MH  - src-Family Kinases/metabolism
EDAT- 2008/05/21 09:00
MHDA- 2008/07/29 09:00
CRDT- 2008/05/21 09:00
PHST- 2007/04/24 00:00 [received]
PHST- 2008/03/04 00:00 [revised]
PHST- 2008/03/20 00:00 [accepted]
PHST- 2008/05/21 09:00 [pubmed]
PHST- 2008/07/29 09:00 [medline]
PHST- 2008/05/21 09:00 [entrez]
AID - S1043-4666(08)00093-8 [pii]
AID - 10.1016/j.cyto.2008.03.010 [doi]
PST - ppublish
SO  - Cytokine. 2008 Jun;42(3):365-71. doi: 10.1016/j.cyto.2008.03.010. Epub 2008 May 
      19.