PMID- 18490844
OWN - NLM
STAT- MEDLINE
DCOM- 20080923
LR  - 20190902
IS  - 0301-4800 (Print)
IS  - 0301-4800 (Linking)
VI  - 54
IP  - 2
DP  - 2008 Apr
TI  - Polypyrimidine tract-binding protein is involved in regulation of albumin 
      synthesis in response to food intake.
PG  - 142-7
AB  - Our recent study demonstrates that polypyrimidine tract-binding protein (PTB), 
      which is a sequence specific RNA-binding protein, attenuates albumin synthesis in 
      a cell-free translation system. In this study, the effects of food intake on 
      regulation of albumin synthesis through binding of PTB to albumin messenger RNA 
      (mRNA) were investigated. Rats were divided into 1 of 3 groups: fed; fasted for 
      36 h; or fasted for 36 h and then refed for 24 h. No significant differences in 
      albumin mRNA levels were found among fed, fasted and refed rats. However, a 
      decrease in the proportion of albumin mRNA associated with polysomes was 
      identified in fasted rats. Furthermore, UV-cross linking analysis demonstrated 
      that levels of albumin mRNA-PTB complex were increased in liver extracts from 
      fasted rats. No significant differences in PTB levels in liver homogenate were 
      found among the experimental groups. However, PTB level in the cytoplasmic 
      fraction was higher in fasted rats than in fed rats. In refed rats, PTB level in 
      the cytoplasmic fraction returned to a level comparable to that in fed rats, but 
      was inhibited by treatment with rapamycin, a mammalian target of rapamycin (mTOR) 
      inhibitor. These results suggest that localization of PTB is regulated by food 
      intake through mTOR signaling, and alterations in level of albumin mRNA-PTB 
      complex play a role in mediating the effects of food intake on albumin synthesis 
      in the rat liver.
FAU - Kuwahata, Masashi
AU  - Kuwahata M
AD  - Department of Molecular Nutrition, Institute of Health Biosciences, The 
      University of Tokushima Graduate School, Tokushima, Japan. kuwahata@kpu.ac.jp
FAU - Kuramoto, Yasuko
AU  - Kuramoto Y
FAU - Sawai, Yukiko
AU  - Sawai Y
FAU - Amano, Saki
AU  - Amano S
FAU - Tomoe, Yuka
AU  - Tomoe Y
FAU - Segawa, Hiroko
AU  - Segawa H
FAU - Tatsumi, Sawako
AU  - Tatsumi S
FAU - Ito, Mikiko
AU  - Ito M
FAU - Kobayashi, Yukiko
AU  - Kobayashi Y
FAU - Kido, Yasuhiro
AU  - Kido Y
FAU - Oka, Tatsuzo
AU  - Oka T
FAU - Miyamoto, Ken-Ichi
AU  - Miyamoto K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Nutr Sci Vitaminol (Tokyo)
JT  - Journal of nutritional science and vitaminology
JID - 0402640
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribosomal Protein S6)
RN  - 0 (Serum Albumin)
RN  - 139076-35-0 (Polypyrimidine Tract-Binding Protein)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Blotting, Northern/methods
MH  - Blotting, Western/methods
MH  - Cytoplasm/metabolism
MH  - Diet
MH  - *Eating
MH  - Fasting
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Insulin/blood
MH  - Liver/drug effects/*metabolism
MH  - Male
MH  - Phosphorylation/drug effects
MH  - Polypyrimidine Tract-Binding Protein/*metabolism
MH  - Polyribosomes/metabolism
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribosomal Protein S6/drug effects/metabolism
MH  - Serum Albumin/biosynthesis/*genetics
MH  - Sirolimus/pharmacology
EDAT- 2008/05/21 09:00
MHDA- 2008/09/24 09:00
CRDT- 2008/05/21 09:00
PHST- 2008/05/21 09:00 [pubmed]
PHST- 2008/09/24 09:00 [medline]
PHST- 2008/05/21 09:00 [entrez]
AID - JST.JSTAGE/jnsv/54.142 [pii]
AID - 10.3177/jnsv.54.142 [doi]
PST - ppublish
SO  - J Nutr Sci Vitaminol (Tokyo). 2008 Apr;54(2):142-7. doi: 10.3177/jnsv.54.142.