PMID- 18491392
OWN - NLM
STAT- MEDLINE
DCOM- 20090831
LR  - 20191210
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 90
IP  - 1
DP  - 2009 Jul
TI  - Periodontal ligament cellular structures engineered with electrospun 
      poly(DL-lactide-co-glycolide) nanofibrous membrane scaffolds.
PG  - 186-95
LID - 10.1002/jbm.a.32066 [doi]
AB  - Periodontal tissue engineering is expected to overcome the limitations associated 
      with the existing regenerative techniques for the treatment of periodontal 
      defects involving alveolar bone, cementum, and periodontal ligament. Cell-based 
      tissue engineering approaches involve the utilization of in vitro expanded cells 
      with regenerative capacity and their delivery to the appropriate sites via 
      biomaterial scaffolds. The aim of this study was to establish living periodontal 
      ligament cell-containing structures on electrospun poly(DL-lactic-co-glycolic 
      acid) (PLGA) nanofiber membrane scaffolds, assess their viability and 
      characteristics, and engineer multilayered structures amenable to easy handling. 
      Human periodontal ligament (hPDL) cells were expanded in explant culture and then 
      characterized morphologically and immunohistochemically. PLGA nanofiber membranes 
      were prepared by the electrospinning process; mechanical tensile properties were 
      determined, surface topography, nanofiber size, and porosity status were 
      investigated with SEM. Cells were seeded on the membranes at approximately 50,000 
      cell/cm(2) and cultured for 21 days either in expansion or in osteogenic 
      induction medium. Cell adhesion and viability were demonstrated using SEM and 
      MTT, respectively, and osteogenic differentiation was determined with IHC and 
      immunohistomorphometric evaluation of osteopontin, osteocalcin, and bone 
      sialoprotein marker expression. At days 3, 6, 9, and 12 additional cell/membrane 
      layers were deposited on the existing ones and multilayered hybrid structures 
      were established. Results indicate the feasibility of periodontal ligament 
      cell-containing tissue-like structures engineering with PDL cells and electrospun 
      nanofiber PLGA scaffolds supporting cell adhesion, viability and osteogenic 
      differentiation properties of cells in hybrid structures amenable to macroscopic 
      handling.
FAU - Inanc, Bulend
AU  - Inanc B
AD  - Tissue Engineering and Biomaterials Laboratory, Ankara University, Faculty of 
      Science and Biotechnology Institute, Ankara 06100, Turkey.
FAU - Arslan, Y Emre
AU  - Arslan YE
FAU - Seker, Sukran
AU  - Seker S
FAU - Elcin, A Eser
AU  - Elcin AE
FAU - Elcin, Y Murat
AU  - Elcin YM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 0 (Biomarkers)
RN  - 34346-01-5 (Polyglactin 910)
SB  - IM
MH  - Biocompatible Materials/chemistry
MH  - Biomarkers/metabolism
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Electrochemistry/*methods
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - Materials Testing
MH  - Nanostructures/*chemistry
MH  - Osteogenesis
MH  - Periodontal Ligament/cytology
MH  - Polyglactin 910/*chemistry
MH  - Stress, Mechanical
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/*chemistry
EDAT- 2008/05/21 09:00
MHDA- 2009/09/01 06:00
CRDT- 2008/05/21 09:00
PHST- 2008/05/21 09:00 [pubmed]
PHST- 2009/09/01 06:00 [medline]
PHST- 2008/05/21 09:00 [entrez]
AID - 10.1002/jbm.a.32066 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2009 Jul;90(1):186-95. doi: 10.1002/jbm.a.32066.