PMID- 1849271
OWN - NLM
STAT- MEDLINE
DCOM- 19910506
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 88
IP  - 7
DP  - 1991 Apr 1
TI  - Cloning of the gamma-aminobutyric acid (GABA) rho 1 cDNA: a GABA receptor subunit 
      highly expressed in the retina.
PG  - 2673-7
AB  - Type A gamma-aminobutyric acid (GABAA) receptors are a family of ligand-gated 
      chloride channels that are the major inhibitory neurotransmitter receptors in the 
      nervous system. Molecular cloning has revealed diversity in the subunits that 
      compose this heterooligomeric receptor, but each previously elucidated subunit 
      displays amino acid similarity in conserved structural elements. We have used 
      these highly conserved regions to identify additional members of this family by 
      using the polymerase chain reaction (PCR). One PCR product was used to isolate a 
      full-length cDNA from a human retina cDNA library. The mature protein predicted 
      from this cDNA sequence in 458 amino acids long and displays between 30 and 38% 
      amino acid similarity to the previously identified GABAA subunits. This gene is 
      expressed primarily in the retina but transcripts are also detected in the brain, 
      lung, and thymus. Injection of Xenopus oocytes with RNA transcribed in vitro 
      produces a GABA-responsive chloride conductance and expression of the cDNA in COS 
      cells yields GABA-displaceable muscimol binding. These features are consistent 
      with our identification of a GABA subunit, GABA rho 1, with prominent retinal 
      expression that increases the diversity and tissue specificity of this 
      ligand-gated ion-channel receptor family.
FAU - Cutting, G R
AU  - Cutting GR
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205.
FAU - Lu, L
AU  - Lu L
FAU - O'Hara, B F
AU  - O'Hara BF
FAU - Kasch, L M
AU  - Kasch LM
FAU - Montrose-Rafizadeh, C
AU  - Montrose-Rafizadeh C
FAU - Donovan, D M
AU  - Donovan DM
FAU - Shimada, S
AU  - Shimada S
FAU - Antonarakis, S E
AU  - Antonarakis SE
FAU - Guggino, W B
AU  - Guggino WB
FAU - Uhl, G R
AU  - Uhl GR
AU  - et al.
LA  - eng
SI  - GENBANK/D90291
SI  - GENBANK/D90292
SI  - GENBANK/D90293
SI  - GENBANK/D90294
SI  - GENBANK/D90295
SI  - GENBANK/D90296
SI  - GENBANK/D90297
SI  - GENBANK/D90298
SI  - GENBANK/M62321
SI  - GENBANK/M62323
SI  - GENBANK/M62400
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Macromolecular Substances)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, GABA-A)
RN  - 124-87-8 (Picrotoxin)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Female
MH  - Gene Library
MH  - Humans
MH  - Macromolecular Substances
MH  - Membrane Potentials/drug effects
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Oocytes/drug effects/physiology
MH  - Organ Specificity
MH  - Picrotoxin/pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Receptors, GABA-A/drug effects/*genetics/physiology
MH  - Retina/*physiology
MH  - Sequence Homology, Nucleic Acid
MH  - Transfection
MH  - Xenopus
MH  - gamma-Aminobutyric Acid/metabolism/pharmacology
PMC - PMC51300
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
PMCR- 1991/10/01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PHST- 1991/10/01 00:00 [pmc-release]
AID - 10.1073/pnas.88.7.2673 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2673-7. doi: 10.1073/pnas.88.7.2673.