PMID- 18493705
OWN - NLM
STAT- MEDLINE
DCOM- 20090106
LR  - 20211020
IS  - 0300-9564 (Print)
IS  - 0300-9564 (Linking)
VI  - 115
IP  - 11
DP  - 2008 Nov
TI  - Role of melatonin receptors in the effects of melatonin on BDNF and 
      neuroprotection in mouse cerebellar neurons.
PG  - 1495-9
LID - 10.1007/s00702-008-0066-z [doi]
AB  - Although melatonin affects developing neurons and is neuroprotective, a role of 
      melatonin receptors termed MT1 and MT2 in these actions is unclear. We 
      investigated the effects of melatonin on the levels of the brain derived 
      neurotrophic factor (BDNF) in the developing cerebellum and cerebellar granule 
      cells (CGC) of wild-type (WT), MT1- and MT2-knockout mice. A model of 
      low-potassium CGC toxicity was used to evaluate neuroprotection. A 14-day-old 
      pups and CGC cultures were treated with melatonin; 0.01 mg/kg intraperitoneally 
      and 1 nM in vitro, respectively. Treatment of WT pups and CGC with melatonin did 
      not alter BDNF levels. The absence of MT2 but not MT1 receptors enabled melatonin 
      to increase cerebellar and CGC BDNF content. Nanomolar melatonin was 
      neuroprotective in MT2-knockout but not WT CGC. We propose that CGC from 
      MT2-knockout mice could serve as a model for studying the influence of melatonin 
      on human CGC, which express MT1 but not MT2 receptors.
FAU - Imbesi, Marta
AU  - Imbesi M
AD  - Department of Psychiatry, The Psychiatric Institute, University of Illinois at 
      Chicago, 1601 West Taylor Street, MC912, Chicago, IL 60612, USA.
FAU - Uz, Tolga
AU  - Uz T
FAU - Manev, Hari
AU  - Manev H
LA  - eng
GR  - R01MH61572/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080521
PL  - Austria
TA  - J Neural Transm (Vienna)
JT  - Journal of neural transmission (Vienna, Austria : 1996)
JID - 9702341
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Melatonin, MT1)
RN  - 0 (Receptor, Melatonin, MT2)
RN  - 0 (Receptors, Melatonin)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EUY85H477I (thiazolyl blue)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Cerebellum/*cytology/drug effects
MH  - Melatonin/*pharmacology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Knockout
MH  - Neurons/*drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptor, Melatonin, MT1/drug effects/genetics
MH  - Receptor, Melatonin, MT2/drug effects/genetics
MH  - Receptors, Melatonin/*drug effects
MH  - Tetrazolium Salts
MH  - Thiazoles
EDAT- 2008/05/22 09:00
MHDA- 2009/01/07 09:00
CRDT- 2008/05/22 09:00
PHST- 2008/03/06 00:00 [received]
PHST- 2008/05/05 00:00 [accepted]
PHST- 2008/05/22 09:00 [pubmed]
PHST- 2009/01/07 09:00 [medline]
PHST- 2008/05/22 09:00 [entrez]
AID - 10.1007/s00702-008-0066-z [doi]
PST - ppublish
SO  - J Neural Transm (Vienna). 2008 Nov;115(11):1495-9. doi: 
      10.1007/s00702-008-0066-z. Epub 2008 May 21.