PMID- 18494052
OWN - NLM
STAT- MEDLINE
DCOM- 20080815
LR  - 20211020
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 14
IP  - 19
DP  - 2008 May 21
TI  - Transplanted bone marrow stromal cells are not cellular origin of hepatocellular 
      carcinomas in a mouse model of carcinogenesis.
PG  - 3015-20
AB  - AIM: To investigate the malignant potential of hepatic stem cells derived from 
      the bone marrow stromal cells (BMSCs) in a mouse model of chemical 
      hepatocarcino-genesis. METHODS: BMSCs from male BALB/c mice were harvested and 
      cultured, then transplanted into female syngenic BALB/c mice via portal vein. 
      Hepato-carcinogenesis was induced by 6 months of treatment with 
      diethylnitrosamine (DEN). Six months later, the liver was removed from each 
      treated mouse and evaluated by immunohistochemistry and fluorescence in situ 
      hybridization (FISH). RESULTS: Twenty-six percent of recipient mice survived and 
      developed multiple hepatocellular carcinomas (HCCs). Immunohistochemically, HCC 
      expressed placental form of glutathione-S-transferase (GST-P) and 
      alpha-fetoprotein, but did not express cytokeratin 19. Y chromosome positive 
      hepatocytes were detected by fluorescent in situ hybridization (FISH) in the 
      liver of mice treated with DEN after BMSCs transplantation while no such 
      hepatocytes were identified in the liver of mice not treated with DEN. No HCC was 
      positive for the Y chromosome by FISH. CONCLUSION: Hepatic stem cells derived 
      from the bone marrow stromal cells have a low malignant potential in our mouse 
      model of chemical hepatocarcingenesis.
FAU - Zheng, Jin-Fang
AU  - Zheng JF
AD  - Department of Hepatobiliary Surgery, the People's Hospital of Hainan Province, 
      Hainan Province, China. zhengjf2000@hotmail.com
FAU - Liang, Li-Jian
AU  - Liang LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (HGF protein, human)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*pathology
MH  - *Bone Marrow Transplantation
MH  - Carcinoma, Hepatocellular/chemically induced/genetics/*pathology
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cell Transformation, Neoplastic/genetics/*pathology
MH  - Cells, Cultured
MH  - Diethylnitrosamine
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Hepatocyte Growth Factor/metabolism
MH  - Hepatocytes/*pathology/transplantation
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms, Experimental/chemically induced/genetics/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplastic Stem Cells/*pathology/transplantation
MH  - Stromal Cells/*pathology/transplantation
MH  - Y Chromosome
PMC - PMC2712168
EDAT- 2008/05/22 09:00
MHDA- 2008/08/16 09:00
PMCR- 2008/05/21
CRDT- 2008/05/22 09:00
PHST- 2008/05/22 09:00 [pubmed]
PHST- 2008/08/16 09:00 [medline]
PHST- 2008/05/22 09:00 [entrez]
PHST- 2008/05/21 00:00 [pmc-release]
AID - 10.3748/wjg.14.3015 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2008 May 21;14(19):3015-20. doi: 10.3748/wjg.14.3015.