PMID- 18494143
OWN - NLM
STAT- MEDLINE
DCOM- 20080708
LR  - 20191027
IS  - 1531-7129 (Print)
IS  - 1531-7129 (Linking)
VI  - 29
IP  - 3
DP  - 2008 Apr
TI  - An analysis of time-dependent changes of neurotrophic factors (BDNF, CNTF) in 
      traumatic facial nerve injury of a nerve-cut and nerve-crush model in rats.
PG  - 392-6
AB  - HYPOTHESIS: To investigate neurotrophic changes in the injured facial nerve in 
      rats. BACKGROUND: Time-dependent follow-up of brain-derived neurotrophic factor 
      (BDNF) and ciliary neurotrophic factor (CNTF) concentrations in a standardized 
      nerve-cut and nerve-crush trauma model could present valuable information about 
      estimation of the extent of the injury. METHODS: Ninety Wistar rats were grouped 
      into permanent (transection: nerve-cut) and transient (compression: nerve-crush) 
      nerve injury and analyzed for neurotrophic changes at Week 1 and after 1, 3, and 
      6 months. Ten undissected rats served as controls. Permanent injury was 
      accomplished with a 5-mm transection. To model transient injury, the facial nerve 
      was pressed by vascular clamps for 40 minutes. Rat BDNF and CNTF enzyme-linked 
      immunosorbent assay kits were used to perform histochemical analysis of blood 
      samples. Measurements were compared with variance analysis and Student's t tests. 
      RESULTS: BDNF levels in nerve-injured preparations from both groups at 
      consecutive intervals were not significant from those of controls, except for 
      transient increases in the first week. When BDNF levels in nerve-cut and 
      nerve-crush groups were compared at each interval separately, differences were 
      insignificant (p > 0.05). CNTF levels at consecutive intervals were significant 
      from that of control rats in both groups. When CN0054F levels in nerve-cut and 
      nerve-crush groups were compared at each interval separately, differences were 
      significant (p = 0.023, p = 0.001, p = 0.043, and p = 0.023). CONCLUSION: 
      Elevated levels of BDNF in the first week after nerve injury do not differentiate 
      the severity of the facial nerve injury. However, diminished CNTF levels in the 
      nerve-crush group were significant when compared with that of nerve-cut group. 
      This finding suggests the possibility that CNTF levels can distinguish whether 
      the facial nerve is interrupted after trauma. Further experimental studies should 
      investigate the indicative role of CNTF levels as a marker of nerve disruption in 
      the clinical setting.
FAU - Yetiser, Sertac
AU  - Yetiser S
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Gulhane Medical 
      School, Etlik, Ankara, Turkey. syetiser@yahoo.com
FAU - Kahraman, Erkan
AU  - Kahraman E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Otol Neurotol
JT  - Otology & neurotology : official publication of the American Otological Society, 
      American Neurotology Society [and] European Academy of Otology and Neurotology
JID - 100961504
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Biomarkers/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Ciliary Neurotrophic Factor/*metabolism
MH  - Disease Models, Animal
MH  - Facial Nerve Injuries/*metabolism
MH  - Male
MH  - Nerve Crush
MH  - Rats
MH  - Rats, Wistar
MH  - *Severity of Illness Index
MH  - Time Factors
EDAT- 2008/05/22 09:00
MHDA- 2008/07/09 09:00
CRDT- 2008/05/22 09:00
PHST- 2008/05/22 09:00 [pubmed]
PHST- 2008/07/09 09:00 [medline]
PHST- 2008/05/22 09:00 [entrez]
AID - 10.1097/mao.0b013e318161ab3e [doi]
PST - ppublish
SO  - Otol Neurotol. 2008 Apr;29(3):392-6. doi: 10.1097/mao.0b013e318161ab3e.