PMID- 18495100
OWN - NLM
STAT- MEDLINE
DCOM- 20080821
LR  - 20220331
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 251
IP  - 2
DP  - 2008 Feb
TI  - Immunological properties of umbilical cord blood-derived mesenchymal stromal 
      cells.
PG  - 116-23
LID - 10.1016/j.cellimm.2008.04.003 [doi]
AB  - Mesenchymal stromal cells (MSCs) are promising candidates for developing cell 
      therapies for intractable diseases. To assess the feasibility of transplantation 
      with human umbilical cord blood (hUCB)-derived MSCs, we analyzed the ability of 
      these cells to function as alloantigen-presenting cells (APC) in vitro. hUCB-MSCs 
      were strongly positive for MSC-related antigens and stained positively for human 
      leukocyte antigen (HLA)-AB and negatively for HLA-DR. When treated with 
      interferon (IFN)-gamma, the expression of HLA-AB and HLA-DR, but not the 
      co-stimulatory molecules CD80 and CD86, was increased. hUCB-MSCs did not provoke 
      allogeneic PBMC (peripheral blood mononuclear cell) proliferation, even when 
      their HLA-molecule expression was up-regulated by IFN-gamma pretreatment. When 
      added to a mixed lymphocyte reaction (MLR), hUCB-MSCs actively suppressed the 
      allogeneic proliferation of the responder lymphocytes. This suppressive effect 
      was mediated by soluble factors. We conclude that hUCB-MSCs can suppress the 
      allogeneic response of lymphocytes and may thus be useful in allogeneic cell 
      therapies.
FAU - Oh, Wonil
AU  - Oh W
AD  - Medipost Biomedical Research Institute, Medipost Co Ltd, Asan Institute of Life 
      Science, Seoul, Republic of Korea.
FAU - Kim, Dal-Soo
AU  - Kim DS
FAU - Yang, Yoon Sun
AU  - Yang YS
FAU - Lee, Jae Kwon
AU  - Lee JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080520
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Differentiation/immunology
MH  - Cytokines/biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Blood/cytology/*immunology
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class I/biosynthesis
MH  - Humans
MH  - Immunophenotyping
MH  - Infant, Newborn
MH  - Interferon-gamma/pharmacology
MH  - Leukocytes, Mononuclear/cytology/drug effects/immunology
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Mesenchymal Stem Cells/cytology/drug effects/*immunology
MH  - Mesoderm
MH  - Pregnancy
MH  - Stromal Cells/cytology/drug effects/immunology
EDAT- 2008/05/23 09:00
MHDA- 2008/08/22 09:00
CRDT- 2008/05/23 09:00
PHST- 2008/01/09 00:00 [received]
PHST- 2008/03/29 00:00 [revised]
PHST- 2008/04/04 00:00 [accepted]
PHST- 2008/05/23 09:00 [pubmed]
PHST- 2008/08/22 09:00 [medline]
PHST- 2008/05/23 09:00 [entrez]
AID - S0008-8749(08)00073-7 [pii]
AID - 10.1016/j.cellimm.2008.04.003 [doi]
PST - ppublish
SO  - Cell Immunol. 2008 Feb;251(2):116-23. doi: 10.1016/j.cellimm.2008.04.003. Epub 
      2008 May 20.