PMID- 18495654
OWN - NLM
STAT- MEDLINE
DCOM- 20081212
LR  - 20211020
IS  - 1464-3553 (Electronic)
IS  - 0379-864X (Print)
IS  - 0379-864X (Linking)
VI  - 33
IP  - 6
DP  - 2008 Jul
TI  - BDNF promoter-mediated beta-galactosidase expression in the olfactory epithelium 
      and bulb.
PG  - 531-9
LID - 10.1093/chemse/bjn021 [doi]
AB  - The neurotrophin brain-derived neurotrophic factor (BDNF) has been implicated in 
      the generation and differentiation of new olfactory sensory neurons (OSNs) and in 
      the regulation of branching of OSN axons in their target glomeruli. However, 
      previous reports of BDNF mRNA and protein expression in olfactory epithelium and 
      olfactory bulb (OB) have been inconsistent, raising questions on the proposed 
      roles for BDNF. Here, we report on beta-galactosidase (beta-gal) expression in 
      adult gene-targeted mice where the BDNF promoter drives expression of the 
      Escherichia coli lacZ gene (BDNF(lacZneo) mice). We find that beta-gal is 
      expressed in a small subset of OSNs with axons that reach the olfactory nerve 
      layers throughout the OB. In the OB, we find expression of beta-gal in 
      gamma-aminobutyric acidergic but not dopaminergic periglomerular cells and 
      external tufted cells and in interneurons located in the mitral cell layer. Our 
      results are inconsistent with the regulation of generation and differentiation of 
      new OSNs elicited by the release of BDNF from horizontal basal cells. The results 
      are consistent with a role for BDNF in competitive branching of OSN axons within 
      the glomeruli of the OB.
FAU - Clevenger, Amy C
AU  - Clevenger AC
AD  - Department of Cell and Developmental Biology, University of Colorado Denver, Mail 
      Stop 8108, PO BOX 6511, Aurora CO 80045, USA.
FAU - Salcedo, Ernesto
AU  - Salcedo E
FAU - Jones, Kevin R
AU  - Jones KR
FAU - Restrepo, Diego
AU  - Restrepo D
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080520
PL  - England
TA  - Chem Senses
JT  - Chemical senses
JID - 8217190
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - *Gene Expression
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Olfactory Bulb/*metabolism
MH  - Olfactory Mucosa/*metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - beta-Galactosidase/*genetics/*metabolism
PMC - PMC2467464
EDAT- 2008/05/23 09:00
MHDA- 2008/12/17 09:00
PMCR- 2008/05/20
CRDT- 2008/05/23 09:00
PHST- 2008/05/23 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/05/23 09:00 [entrez]
PHST- 2008/05/20 00:00 [pmc-release]
AID - bjn021 [pii]
AID - 10.1093/chemse/bjn021 [doi]
PST - ppublish
SO  - Chem Senses. 2008 Jul;33(6):531-9. doi: 10.1093/chemse/bjn021. Epub 2008 May 20.