PMID- 1849670
OWN - NLM
STAT- MEDLINE
DCOM- 19910513
LR  - 20190818
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 66
IP  - 2
DP  - 1991 Feb
TI  - Presence of the 4 S polycyclic hydrocarbon-binding protein in H4-II-E cells.
PG  - 175-86
AB  - The 4 S polycyclic aromatic hydrocarbon (PAH)-binding protein had been implicated 
      in regulating the expression of rat cytochrome P450IA1 which is most closely 
      associated with aryl hydrocarbon hydroxylase (AHH). We have now investigated the 
      presence of both the 4 S PAH-binding protein and the 8 S Ah receptor in rat 
      hepatoma H4-II-E cells as well as the induction of P450IA1 upon their exposure to 
      PAH's such as benzo[a]pyrene (BP) and 3-methylcholanthrene (3MC), and halogenated 
      dioxins such as 2,3,7,8-tetrachlordibenzo-p-dioxin (TCDD) and 
      2,3,7,8-tetrachlorodibenzofuran (TCDBF). Sucrose density gradient analyses and 
      hydroxylapatite assays indicate that, in addition to the 8 S protein, the 4 S PAH 
      binding protein is present in these cells. This protein interacts in a saturable 
      and high affinity manner with BP and 3MC, but not with TCDD or TCDBF. Using a 
      P450IA1 probe, the induction of gene expression was observed by Northern blot 
      analysis of total cellular RNA after exposure of the H4-II-E cells to BP, 3MC, or 
      TCDBF. Since the 4 S protein was observed to interact only with BP and 3MC, these 
      results suggest that this protein may also play a role in the PAH-induced 
      expression of cytochrome P450IA1 gene expression in H4-II-E.
FAU - Raha, A
AU  - Raha A
AD  - Eppley Institute for Research in Cancer and Allied Diseases, University of 
      Nebraska Medical Center, Omaha 68105.
FAU - Reddy, V
AU  - Reddy V
FAU - Xu, L C
AU  - Xu LC
FAU - Houser, W H
AU  - Houser WH
FAU - Bresnick, E
AU  - Bresnick E
LA  - eng
GR  - CA 36106/CA/NCI NIH HHS/United States
GR  - ES 03980/ES/NIEHS NIH HHS/United States
GR  - T32-CA 09476/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Carrier Proteins)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Receptors, Drug)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 56-49-5 (Methylcholanthrene)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.20 (Glycine N-Methyltransferase)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/*toxicity
MH  - Carrier Proteins/*metabolism
MH  - Cytochrome P-450 Enzyme System/*genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Glycine N-Methyltransferase
MH  - Liver Neoplasms, Experimental/enzymology/*metabolism
MH  - Methylcholanthrene/*toxicity
MH  - *Methyltransferases
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Protein Binding/drug effects
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Aryl Hydrocarbon
MH  - Receptors, Drug/*drug effects/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
AID - 0300-483X(91)90217-O [pii]
AID - 10.1016/0300-483x(91)90217-o [doi]
PST - ppublish
SO  - Toxicology. 1991 Feb;66(2):175-86. doi: 10.1016/0300-483x(91)90217-o.