PMID- 18497950
OWN - NLM
STAT- MEDLINE
DCOM- 20080807
LR  - 20080523
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 19
IP  - 6
DP  - 2008 Jun
TI  - HLA-B gene participates in the NF-kappaB signal pathway partly by regulating 
      S100A8 in the laryngeal carcinoma cell line Hep2.
PG  - 1453-9
AB  - Human leukocyte antigen B (HLA-B), a novel member of the NF-kappaB signal pathway 
      in laryngeal squamous cell carcinoma (LSCC), mediates immunological surveillance 
      of tumor cells by presenting peptides to cytotoxic T-lymphocytes (CTLs) together 
      with S100 calcium binding protein A8 (S100A8). The objective of this study was to 
      investigate the molecular mechanism of HLA-B and S100A8 in laryngeal 
      carcinogenesis. Flow cytometry, 3-(4,5-dimethyl-2-thiazolyl)-2, 
      5-diphenyl-2H-tetrazolium bromide (MTT) assay and cell invasion in vitro were 
      used to detect the biological effect of the Hep2 cell line induced by HLA-B RNA 
      interference. RT-PCR and Western blotting were applied to evaluate the expression 
      level of the S100A8 gene after HLA-B RNA interference. Our results showed that 
      HLA-B had negative effects on Hep2 cells by inhibiting apoptosis and cell 
      invasion while decreasing cell proliferation. Additionally, the expression level 
      of HLA-B and S100A8 in LSCC were down-regulated after HLA-B RNA interference. The 
      abnormal expression of HLA-B is thus relevant to the biological effect of 
      laryngeal carcinoma and participates in the NF-kappaB signal pathway partly by 
      regulating the expression of the S100A8 gene.
FAU - Guo, Yan
AU  - Guo Y
AD  - Department of Medical Genetics, China Medical University, Shenyang, PR China.
FAU - Liu, Jia
AU  - Liu J
FAU - Xu, Zhenming
AU  - Xu Z
FAU - Sun, Kailai
AU  - Sun K
FAU - Fu, Weineng
AU  - Fu W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Calgranulin A)
RN  - 0 (HLA-B Antigens)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Blotting, Western
MH  - Calgranulin A/*metabolism
MH  - Cell Cycle
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - HLA-B Antigens/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Mice
MH  - NF-kappa B/*metabolism
MH  - NIH 3T3 Cells
MH  - Neoplasm Invasiveness
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Signal Transduction
EDAT- 2008/05/24 09:00
MHDA- 2008/08/08 09:00
CRDT- 2008/05/24 09:00
PHST- 2008/05/24 09:00 [pubmed]
PHST- 2008/08/08 09:00 [medline]
PHST- 2008/05/24 09:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2008 Jun;19(6):1453-9.